Overview

  • Product name

    Anti-PAR6 antibody [EPR12378] - N-terminal
    See all PAR6 primary antibodies
  • Description

    Rabbit monoclonal [EPR12378] to PAR6 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PAR6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NPB6

  • Positive control

    • HeLa, 293T, Jurkat, Y79 and fetal liver lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
    • Tissue specificity

      Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
    • Sequence similarities

      Belongs to the PAR6 family.
      Contains 1 OPR domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 pseudo-CRIB domain.
    • Domain

      The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
      The OPR domain mediates interactions with MAP2K5.
      The PDZ domain mediates the interaction with CRB3.
    • Cellular localization

      Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
    • Information by UniProt
    • Database links

    • Alternative names

      • 0710008C04Rik antibody
      • 2610010A15Rik antibody
      • Par 6 partitioning defective 6 C elegans homolog alpha antibody
      • Par 6 partitioning defective 6 homolog alpha antibody
      • Par 6 partitioning defective 6 homolog alpha C elegans antibody
      • PAR 6A antibody
      • PAR-6 alpha antibody
      • PAR-6 antibody
      • par-6 family cell polarity regulator alpha antibody
      • PAR-6A antibody
      • PAR6 antibody
      • PAR6A_HUMAN antibody
      • PAR6alpha antibody
      • PAR6C antibody
      • Pard6a antibody
      • Partitioning defective 6 homolog alpha antibody
      • partitioning-defective protein 6 antibody
      • partitioning-defective protein 6, C. elegans, homolog of, alpha antibody
      • Tax interacting protein 40 antibody
      • Tax interaction protein 40 antibody
      • TAX40 antibody
      • TIP 40 antibody
      • TIP-40 antibody
      • TIP40 antibody
      see all

    Images

    • All lanes : Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 37 kDa

    • Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    • Johnson MB  et al. Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature 556:370-375 (2018). Read more (PubMed: 29643508) »
    • Zuckerwise L  et al. H19 long noncoding RNA alters trophoblast cell migration and invasion by regulating TßR3 in placentae with fetal growth restriction. Oncotarget 7:38398-38407 (2016). Read more (PubMed: 27223264) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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