Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12378] to PAR6 - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-PAR6 antibody [EPR12378] - N-terminal
See all PAR6 primary antibodies
DescriptionRabbit monoclonal [EPR12378] to PAR6 - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PAR6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NPB6
- HeLa, 293T, Jurkat, Y79 and fetal liver lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Tissue specificityExpressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
Sequence similaritiesBelongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain.
DomainThe pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3.
Cellular localizationCytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
- Information by UniProt
- 0710008C04Rik antibody
- 2610010A15Rik antibody
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
All lanes : Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab180159 has been referenced in 2 publications.
- Johnson MB et al. Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature 556:370-375 (2018). PubMed: 29643508
- Zuckerwise L et al. H19 long noncoding RNA alters trophoblast cell migration and invasion by regulating TßR3 in placentae with fetal growth restriction. Oncotarget 7:38398-38407 (2016). PubMed: 27223264