Overview

  • Product name

    Paraoxonase 1 Activity Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma
  • Product overview

    Paraoxonase 1 Activity Assay Kit (ab241044) enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). This ensures dramatically greater sensitivity than UV or colorimetric assays and eliminates the need for dangerous toxic substrates. A selective PON1 inhibitor is provided for verification of PON1 specific activity. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 2.0 μU paraoxonase activity with a sample volume of 5 μL.

  • Platform

    Microplate reader

Properties

Images

  • Standard curve of PON1 Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of PON1 Substrate.
  • Reaction kinetics of PON1 Substrate metabolism in donor-pooled human serum (5 µL), donor-pooled human plasma (5 µL) and rabbit serum (2.5 µL) in the presence and absence of 200 µM of the selective PON1 inhibitor 2- hydroxyquinoline (no inhibitor conditions contained 0.4% DMSO as a solvent control).

  • Quantification of PON1 activity in serum/plasma samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.

Protocols

References

ab241044 has not yet been referenced specifically in any publications.

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