Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8481] to Parathyroid Hormone
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
Product nameAnti-Parathyroid Hormone antibody [EPR8481]
See all Parathyroid Hormone primary antibodies
DescriptionRabbit monoclonal [EPR8481] to Parathyroid Hormone
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human Parathyroid Hormone was used as an immunogen (UniProt ID: P01270). (The amino acid sequence is considered to be commercially sensitive).
- Recombinant protein; Human parathyroid gland tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 13 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
Sequence similaritiesBelongs to the parathyroid hormone family.
- Information by UniProt
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
Anti-Parathyroid Hormone antibody [EPR8481] (ab166632) at 1/1000 dilution + 10ng of tagged recombinant Human Parathyroid Hormone
Predicted band size: 13 kDa
Direct ELISA antibody dose-response curve using recombinant anti-Human Parathyroid Hormone antibody (ab166632) against full length human parathyroid hormone protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody.
Antigen concentration: 100 ng/ml
Primary ab concentration: 0-1000 ng/ml
Secondary ab concentration: 1:200 dilution
Immunohistochemical analysis of paraffin embedded Human parathyroid gland tissue labeling Parathyroid Hormone with ab166632 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab166632 has not yet been referenced specifically in any publications.