Recombinant
RabMAb

Recombinant Anti-Parathyroid Hormone antibody [EPR8481] (ab166632)

Overview

  • Product name

    Anti-Parathyroid Hormone antibody [EPR8481]
    See all Parathyroid Hormone primary antibodies
  • Description

    Rabbit monoclonal [EPR8481] to Parathyroid Hormone
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Parathyroid Hormone was used as an immunogen (UniProt ID: P01270). (The amino acid sequence is considered to be commercially sensitive).

  • Positive control

    • Recombinant protein; Human parathyroid gland tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8481
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
    • Involvement in disease

      Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
    • Sequence similarities

      Belongs to the parathyroid hormone family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • hPTH antibody
      • Parathormone antibody
      • Parathyrin antibody
      • Parathyroid hormone 1 antibody
      • Parathyroid hormone antibody
      • Prepro PTH antibody
      • Preproparathyroid hormone antibody
      • PTH antibody
      • PTH1 antibody
      • PTH1 receptor antibody
      • PTH1R antibody
      • PTHR antibody
      • PTHR1 antibody
      • PTHY_HUMAN antibody
      see all

    Images

    • Anti-Parathyroid Hormone antibody [EPR8481] (ab166632) at 1/1000 dilution + 10ng of tagged recombinant Human Parathyroid Hormone

      Predicted band size: 13 kDa

    • Direct ELISA antibody dose-response curve using recombinant anti-Human Parathyroid Hormone antibody (ab166632) against full length human parathyroid hormone protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody.

      Antigen concentration: 100 ng/ml

      Primary ab concentration: 0-1000 ng/ml

      Secondary ab concentration: 1:200 dilution

    • Immunohistochemical analysis of paraffin embedded Human parathyroid gland tissue labeling Parathyroid Hormone with ab166632 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab166632 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab166632.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up