Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9939] to Parathyroid Hormone
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
Product nameAnti-Parathyroid Hormone antibody [EPR9939]
See all Parathyroid Hormone primary antibodies
DescriptionRabbit monoclonal [EPR9939] to Parathyroid Hormone
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Parathyroid Hormone.
- Tagged Parathyroid Hormone recombinant protein; Human parathyroid gland carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/50000 - 1/200000. Predicted molecular weight: 13 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
Sequence similaritiesBelongs to the parathyroid hormone family.
- Information by UniProt
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
Anti-Parathyroid Hormone antibody [EPR9939] (ab154792) at 1/50000 dilution + Tagged Parathyroid Hormone recombinant protein at 0.01 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 13 kDa
Direct ELISA antibody dose-response curve using recombinant anti-Human Parathyroid Hormone antibody (ab154792) against full length human parathyroid hormone protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody.
Antigen concentration: 100 ng/ml
Primary ab concentration: 0-1000 ng/ml
Secondary ab concentration: 1:200 dilution
Immunohistochemical analysis of paraffin-embedded Human parathyroid gland carcinoma tissue labeling Parathyroid Hormone with ab154792 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab154792 has been referenced in 1 publication.
- Pan J et al. Regeneration of a Bioengineered Thyroid Using Decellularized Thyroid Matrix. Thyroid 29:142-152 (2019). PubMed: 30375266