Recombinant Anti-Parathyroid Hormone antibody [EPR9939] - BSA and Azide free (ab221800)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9939] to Parathyroid Hormone - BSA and Azide free
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Parathyroid Hormone antibody [EPR9939] - BSA and Azide free
See all Parathyroid Hormone primary antibodies -
Description
Rabbit monoclonal [EPR9939] to Parathyroid Hormone - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment within Human Parathyroid Hormone. The exact sequence is proprietary.
Database link: P01270 -
Positive control
- Tagged Parathyroid Hormone recombinant protein; Human parathyroid gland carcinoma tissue.
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General notes
ab221800 is the carrier-free version of ab154792.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9939 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. -
Involvement in disease
Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. -
Sequence similarities
Belongs to the parathyroid hormone family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5741 Human
- Omim: 168450 Human
- SwissProt: P01270 Human
- Unigene: 37045 Human
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Alternative names
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
see all
Images
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Anti-Parathyroid Hormone antibody [EPR9939] (ab154792) at 1/50000 dilution + Tagged Parathyroid Hormone recombinant protein at 0.01 µg
Secondary
Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 13 kDaThis data was developed using ab154792, the same antibody clone in a different buffer formulation.
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This data was developed using ab154792, the same antibody clone in a different buffer formulation.Direct ELISA antibody dose-response curve using recombinant anti-Human Parathyroid Hormone antibody (ab154792) against full length human parathyroid hormone protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody. Antigen concentration: 100 ng/ml Primary ab concentration: 0-1000 ng/ml Secondary ab concentration: 1:200 dilution
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This data was developed using ab154792, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human parathyroid gland carcinoma tissue labeling Parathyroid Hormone with ab154792 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab221800 has not yet been referenced specifically in any publications.