Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP151] to Parathyroid Hormone - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-Parathyroid Hormone antibody [SP151] - BSA and Azide free
See all Parathyroid Hormone primary antibodies
DescriptionRabbit monoclonal [SP151] to Parathyroid Hormone - BSA and Azide free
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Monkey
Synthetic peptide within Human Parathyroid Hormone (internal sequence). The exact sequence is proprietary.
Database link: P01270
- IHC-P: Human parathyroid gland tissue.
FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Ab236229 is the carrier-free version of ab130759. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab236229 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab236229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
Sequence similaritiesBelongs to the parathyroid hormone family.
- Information by UniProt
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human parathyroid gland tissue sections labeling Parathyroid Hormone with ab130759 at 1/100 dilution (5.77 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human parathyroid gland, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab130759 for 10 mins at room temperature. This image was generated using ab130759, the same clone, but with a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236229 has not yet been referenced specifically in any publications.