The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Expressed in most tissues. Most abundant in kidney, bone and liver.
Involvement in disease
Jansen metaphyseal chondrodysplasia Chondrodysplasia Blomstrand type Enchondromatosis multiple Eiken skeletal dysplasia Primary failure of tooth eruption
Belongs to the G-protein coupled receptor 2 family.