Overview

  • Product name

    Anti-Parathyroid Hormone Receptor 1/PTH1R antibody
    See all Parathyroid Hormone Receptor 1/PTH1R primary antibodies
  • Description

    Rabbit polyclonal to Parathyroid Hormone Receptor 1/PTH1R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Lizard
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Parathyroid Hormone Receptor 1/PTH1R (internal sequence).

  • Positive control

    • Human brain tissue. Extracts from Jurkat cells. MCF7 cells.
  • General notes

     This product was previously labelled as Parathyroid Hormone Receptor 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 66 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in most tissues. Most abundant in kidney, bone and liver.
  • Involvement in disease

    Jansen metaphyseal chondrodysplasia
    Chondrodysplasia Blomstrand type
    Enchondromatosis multiple
    Eiken skeletal dysplasia
    Primary failure of tooth eruption
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC138426 antibody
    • MGC138452 antibody
    • Parathyroid hormone 1 receptor antibody
    • Parathyroid hormone/parathyroid hormone related peptide receptor antibody
    • Parathyroid hormone/parathyroid hormone related protein receptor antibody
    • Parathyroid hormone/parathyroid hormone-related peptide receptor antibody
    • PTH receptor antibody
    • PTH/PTHr receptor antibody
    • PTH/PTHrP receptor antibody
    • PTH/PTHrP type I receptor antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTH1R_HUMAN antibody
    • PTHR 1 antibody
    • PTHR antibody
    • PTHR1 antibody
    see all

Images

  • All lanes : Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 66 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 117 kDa, 82 kDa. We are unsure as to the identity of these extra bands.

  • ab75150 staining Parathyroid Hormone Receptor 1/PTH1R in Lizard regenerated tail (Anolis sagrei) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 45 minutes at 22°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in 1% horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    Image is of a regenerated lizard tail cartilage tube, at a proximal region that has begun to ossify. Positive staining was observed in structures resembling embryonic bone collar and early hypertrophic chondrocytes.

    See Abreview

  • Immunohistochemistry analysis of Parathyroid Hormone Receptor 1/PTH1R in paraffin-embedded human brain tissue using ab75150, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

  • Immunofluorescence analysis of Parathyroid Hormone Receptor 1/PTH1R in MCF7 cells using ab75150, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References

This product has been referenced in:

  • Chen S  et al. Preactivation of ß-catenin in osteoblasts improves the osteoanabolic effect of PTH in type 1 diabetic mice. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 31301073) »
  • He S  et al. Parathyroid Hormone-Like Hormone Induces Epithelial-to-Mesenchymal Transition of Intestinal Epithelial Cells by Activating the Runt-Related Transcription Factor 2. Am J Pathol 188:1374-1388 (2018). Read more (PubMed: 29577935) »
See all 6 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (osteoblasts)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
osteoblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 25 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney, Liver)
Permeabilization
No
Specification
Kidney, Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Daniel Tausan

Verified customer

Submitted Mar 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Lizard Tissue sections (Regenerated lizard tail (Anolis sagrei))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer, pH 6.0
Permeabilization
No
Specification
Regenerated lizard tail (Anolis sagrei)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Dr. Thomas Lozito

Verified customer

Submitted May 06 2014

Question
Answer

Thank you for your phone call today and for your questions about using ab75150 in IHC.

Please keep me updated about the results using this antibody after a heat-mediated antigen retrieval with pH 6 citrate buffer. If you need any further assistance, please don't hesitate to contact me.

Please let me know if you have any questions or if there is anything else that we can do for you.

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Answer

ab12008 : DISCOUNT CODE: *********** Expiration date: DD MM YYYY ab75150 : DISCOUNT CODE: *********** Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test both ab12008 and ab75150 in Rat. Each code will give your customer 1 free primary before the expiration date. To redeem this offer, please submit an Abreview for Rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

DISCOUNT CODE: ********Expiration date: 20April 2012 I am very pleased to hear you would like to accept our offer and test ab54186 in cow tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for cow tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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