Recombinant
RabMAb

Recombinant Anti-Parathyroid Hormone Receptor 1/PTH1R antibody [EPR15892] (ab189924)

Overview

  • Product name

    Anti-Parathyroid Hormone Receptor 1/PTH1R antibody [EPR15892]
    See all Parathyroid Hormone Receptor 1/PTH1R primary antibodies
  • Description

    Rabbit monoclonal [EPR15892] to Parathyroid Hormone Receptor 1/PTH1R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Parathyroid Hormone Receptor 1/PTH1R aa 50-200. The exact sequence is proprietary.
    Database link: Q03431

  • Positive control

    • HeLa and Saos2 lysates.
  • General notes

     

     

     This product was previously labelled as Parathyroid Hormone Receptor 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15892
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/70.
WB 1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).

Target

  • Function

    Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in most tissues. Most abundant in kidney, bone and liver.
  • Involvement in disease

    Jansen metaphyseal chondrodysplasia
    Chondrodysplasia Blomstrand type
    Enchondromatosis multiple
    Eiken skeletal dysplasia
    Primary failure of tooth eruption
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC138426 antibody
    • MGC138452 antibody
    • Parathyroid hormone 1 receptor antibody
    • Parathyroid hormone/parathyroid hormone related peptide receptor antibody
    • Parathyroid hormone/parathyroid hormone related protein receptor antibody
    • Parathyroid hormone/parathyroid hormone-related peptide receptor antibody
    • PTH receptor antibody
    • PTH/PTHr receptor antibody
    • PTH/PTHrP receptor antibody
    • PTH/PTHrP type I receptor antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTH1R_HUMAN antibody
    • PTHR 1 antibody
    • PTHR antibody
    • PTHR1 antibody
    see all

Images

  • Anti-Parathyroid Hormone Receptor 1/PTH1R antibody [EPR15892] (ab189924) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Anti-Parathyroid Hormone Receptor 1/PTH1R antibody [EPR15892] (ab189924) at 1/1000 dilution + Saos2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Western blot analysis of Parathyroid Hormone Receptor 1/PTH1R in HeLa lysate immunoprecipitated using ab189924 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (Preosteoblasts/osteoblasts)
Permeabilization
No
Gating Strategy
Shift in FITC-A
Specification
Preosteoblasts/osteoblasts
Preparation
Cell harvesting/tissue preparation method: Live cells--accutase removal from flask
Sample buffer: PBS + 0.5% BSA and 2mM EDTA
Fixation
Live cells

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Verified customer

Submitted Apr 13 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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