Product nameAnti-PARD6G antibody
DescriptionRabbit polyclonal to PARD6G
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human PARD6G aa 249-326.
Database link: Q9BYG4
- WB: Mouse heart, kidney, brain and skeletal muscle lysates. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notes>95% pure
Our Abpromise guarantee covers the use of ab223049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41, 13 kDa).|
|ICC/IF||1/50 - 1/200.|
RelevanceAdapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
- PAR 6 gamma protein antibody
- par 6 partitioning defective 6 homolog gamma antibody
- PAR 6G antibody
All lanes : Anti-PARD6G antibody (ab223049) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse skeletal muscle lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 41, 13 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PARD6G (green) using ab223049 at 1/100 dilution in ICC/IF.
ab223049 has not yet been referenced specifically in any publications.