Overview

  • Product name

  • Description

    Mouse polyclonal to PARG
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Full length protein, corresponding to amino acids 1-976 of Human PARG (NP_003622.2, UniProt ID: Q86W56-1)

  • Positive control

    • Recombinant Human PARG protein (ab152879) can be used as a positive control in WB. PARG-transfected 293T cell lysate; HeLa nuclear extract; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Anti-PARG antibody (ab169639) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 111 kDa

  • All lanes : Anti-PARG antibody (ab169639) at 1 µg/ml

    Lane 1 : PARG-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 111 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling PARG using ab169639 at 10 µg/ml.

References

This product has been referenced in:

  • Karpova Y  et al. Non-NAD-like PARP-1 inhibitors in prostate cancer treatment. Biochem Pharmacol N/A:N/A (2019). Read more (PubMed: 30880062) »
  • Wang JQ  et al. PARG regulates the proliferation and differentiation of DCs and T cells via PARP/NF-?B in tumour metastases of colon carcinoma. Oncol Rep 41:2657-2666 (2019). Read more (PubMed: 30864743) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab169639.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up