Overview

  • Product name

  • Description

    Rabbit polyclonal to PARG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PARG aa 250-300. The exact sequence is proprietary.
    Sequence:

    CQQDEIDVVPE SPLSDVGSED VGTGPKNDNK LTRQESCLGN SPPFEKESEP


    Database link: Q86W56

  • Positive control

    • 293T whole cell lysate.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab217129 was affinity purified using an epitope specific to PARG immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the poly(ADP-ribose) glycohydrolase family.
    • Cellular localization

      Cytoplasm and Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADP riboseglycohydrolase antibody
      • Mitochondrial poly(ADP ribose) glycohydrolase antibody
      • Parg antibody
      • PARG_HUMAN antibody
      • PARG99 antibody
      • Poly antibody
      • poly (ADP ribose) glycohydrolase antibody
      • poly(ADP-ribose) glycohydrolase 60 kDa isoform antibody
      • Poly(ADP-ribose) glycohydrolase antibody
      see all

    Images

    • Detection of PARG in Immunoprecipitates of 293Twhole cell lysates (1 mg for IP, 20% of IP loaded) using ab217129 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217129 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab217129 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab217129.
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