Product nameAnti-PARIS antibody
DescriptionRabbit polyclonal to PARIS
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Rabbit, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to Human PARIS aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse Brain tissue lysate. IHC-P: Rat brain FFPE tissue sections
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab130867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent - 3%.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 KRAB domain.
modificationsUbiquitinated by PARK2. 'Lys-48'-linked polyubiquitination by PARK2 leads to degradation by the proteasome and may play a key role in regulation of neuron death.
Cellular localizationCytoplasm. Nucleus. Mainly localizes to the cytoplasm; probably translocates to the nucleus to repress selected genes.
- Information by UniProt
- PARIS antibody
- Parkin-interacting substrate antibody
- Zinc finger protein 746 antibody
All lanes : Anti-PARIS antibody (ab130867) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Human PARIS peptide (ab157686) at 1 µg/ml
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Anti-PARIS antibody (ab130867) detects PARIS protein at ~100kDa, consistent with published data (PMID:21376232). This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab130867 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
IHC image of PARIS staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130867, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.