Overview

  • Product name

    Anti-PARIS antibody
  • Description

    Rabbit polyclonal to PARIS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human PARIS aa 300-400 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab157686)

  • Positive control

    • This antibody gave a positive signal in Mouse Brain tissue lysate. IHC-P: Rat brain FFPE tissue sections

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent - 3%.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Post-translational
    modifications

    Ubiquitinated by PARK2. 'Lys-48'-linked polyubiquitination by PARK2 leads to degradation by the proteasome and may play a key role in regulation of neuron death.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly localizes to the cytoplasm; probably translocates to the nucleus to repress selected genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • PARIS antibody
    • Parkin-interacting substrate antibody
    • Zinc finger protein 746 antibody
    • ZN746_HUMAN antibody
    • ZNF746 antibody
    see all

Images

  • All lanes : Anti-PARIS antibody (ab130867) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate with Human PARIS peptide (ab157686) at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 102 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    Anti-PARIS antibody (ab130867) detects PARIS protein at ~100kDa, consistent with published data (PMID:21376232). This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab130867 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • IHC image of PARIS staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130867, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
BRAIN
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 06 2016

Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine gel)
Sample
Human Cell lysate - whole cell (SHSY5Y, N2a, HT22)
Specification
SHSY5Y, N2a, HT22
Blocking step
Odyssey blocking soln as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 37°C

Kalpita Banerjee

Verified customer

Submitted Sep 05 2013

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