Anti-PARIS antibody (ab130867)
Key features and details
- Rabbit polyclonal to PARIS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PARIS antibody -
Description
Rabbit polyclonal to PARIS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human PARIS aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab157686) -
Positive control
- This antibody gave a positive signal in Mouse Brain tissue lysate. IHC-P: Rat brain FFPE tissue sections
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent - 3%.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent - 3%. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
Post-translational
modificationsUbiquitinated by PARK2. 'Lys-48'-linked polyubiquitination by PARK2 leads to degradation by the proteasome and may play a key role in regulation of neuron death. -
Cellular localization
Cytoplasm. Nucleus. Mainly localizes to the cytoplasm; probably translocates to the nucleus to repress selected genes. - Information by UniProt
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Database links
- Entrez Gene: 155061 Human
- Entrez Gene: 69228 Mouse
- Entrez Gene: 312303 Rat
- Omim: 613914 Human
- SwissProt: Q6NUN9 Human
- SwissProt: Q3U133 Mouse
- Unigene: 24643 Human
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Alternative names
- PARIS antibody
- Parkin-interacting substrate antibody
- Zinc finger protein 746 antibody
see all
Images
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All lanes : Anti-PARIS antibody (ab130867) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Human PARIS peptide (ab157686) at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Anti-PARIS antibody (ab130867) detects PARIS protein at ~100kDa, consistent with published data (PMID:21376232). This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab130867 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
IHC image of PARIS staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130867, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab130867 has been referenced in 4 publications.
- Chiang S et al. Mechanisms of impaired mitochondrial homeostasis and NAD+ metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation. Redox Biol 46:102038 (2021). PubMed: 34416478
- Bae JH et al. ZNF746/PARIS overexpression induces cellular senescence through FoxO1/p21 axis activation in myoblasts. Cell Death Dis 11:359 (2020). PubMed: 32398756
- Chen CCW et al. Role of Parkin and endurance training on mitochondrial turnover in skeletal muscle. Skelet Muscle 8:10 (2018). PubMed: 29549884
- Chen CCW et al. Parkin is required for exercise-induced mitophagy in muscle: impact of aging. Am J Physiol Endocrinol Metab 315:E404-E415 (2018). PubMed: 29812989