Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)
![Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)](https://www.abcam.com/ps/products/284/ab284751/Images/ab284751-476434-anti-park7dj1-Wildtype-HEK293T-Human-epithelial-cell-line-from-embryonic-kidney-transformed-with-large-T-antigen-whole-cell-lysate-antibody-ep2816y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)")
Key features and details
- Rabbit monoclonal [EP2816Y] to PARK7/DJ1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free
See all PARK7/DJ1 primary antibodies -
Description
Rabbit monoclonal [EP2816Y] to PARK7/DJ1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TF-1, Jurkat, HEK293T, HAP1 and HeLa cell lysates; Human brain nuclear extract tissue lysate; Human brain tissue lysate. Flow Cyt (intra): Jurkat cells. IHC-P: Human brain tissue.
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General notes
ab284751 is the carrier-free version of ab76241
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2816Y -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.
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| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa. |
Target
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Function
Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone. -
Tissue specificity
Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. -
Involvement in disease
Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease). -
Sequence similarities
Belongs to the peptidase C56 family. -
Post-translational
modificationsSumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
Cys-106 is easily oxidized to sulfinic acid.
Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. -
Cellular localization
Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients. - Information by UniProt
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Database links
- Entrez Gene: 11315 Human
- Omim: 602533 Human
- SwissProt: Q99497 Human
- Unigene: 419640 Human
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Alternative names
- CAP1 antibody
- DJ-1 antibody
- DJ1 antibody
see all
Images
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Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T Human epithelial cell line from embryonic kidney transformed with large T antigen whole cell lysate
Lane 2 : PARK7 knockout HEK-293T Human epithelial cell line from embryonic kidney transformed with large T antigen whole cell lysate
Lane 3 : HeLa Human epithelial cell line from cervix adenocarcinoma whole cell lysate
Lane 4 : Human brain nuclear fraction tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using ab76241, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab76241 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.
ab76241 Anti-PARK7/DJ1 antibody [EP2816Y] was shown to specifically react with PARK7/DJ1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266338 (knockout cell lysate ab257016) was used. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab76241 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)](https://www.abcam.com/ps/products/284/ab284751/Images/ab284751-476432-anti-park7-NULL-dj1-antibody-ep2816y-western-blot.jpg)
Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PARK/DJ1 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using ab76241, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PARK/DJ1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76241 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76241 was shown to specifically react with PARK7/DJ1 in wild-type HAP1 cells. No band was observed when PARK7/DJ1 knockout samples were used. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab76241 and ab8245 (loading control to PARK7/DJ1) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
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![Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)](https://www.abcam.com/ps/products/284/ab284751/Images/ab284751-476433-anti-park7-TF1-cell-lysate-dj1-antibody-ep2816y-western-blot.gif)
Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/20000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using ab76241, the same antibody clone in a different buffer formulation.
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![Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)](https://www.abcam.com/ps/products/284/ab284751/Images/ab284751-2-anti-park7-dj1-AQ73antibody-ep2816y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)This data was developed using ab76241, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab76241 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76241, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)](https://www.abcam.com/ps/products/284/ab284751/Images/ab284751-3-anti-park7-dj1-AQ73antibody-ep2816y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] - BSA and Azide free (ab284751)This data was developed using ab76241, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human brain tissue using ab76241 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab284751 has not yet been referenced specifically in any publications.
