Application
Western blot
Sample
Human Cell lysate - other (head and neck cancer)
Gel Running Conditions
Reduced Denaturing (12% SDS page gel)
Loading amount
30 µg
Treatment
5uM DIM-C-pPhtBu for 24h
Specification
head and neck cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in Skim milk(in PBST)
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody (cell signalin
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL™ Select Western Blotting Detection R
Exposure
30 second(s)
Bands
Specific: 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sung Un Kang
Verified customer
Submitted Sep 18 2018