Application
Western blot
Sample
Human Cell lysate - whole cell (293T, ARPE-19)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris Gel)
Loading amount
10 µg
Specification
293T, ARPE-19
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: ECL Mouse IgG, HRP-linked whole Ab from sheep
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 27, 76 kDa
Additional data
Additional Notes
1-293T.
2-ARPE19.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jul 24 2019