Product nameParkinson's Disease Research Antibody Panel
ab226767 is a Parkinson's Disease research panel containing 5 recombinant rabbit monoclonal antibodies and 1 Anti-Rabbit IgG (HRP) secondary antibody: Anti-alpha synuclein antibody, Anti-LRRK2 antibody, Anti-PINK1 antibody, Anti-PARK/DJ1 antibody and Goat Anti-Rabbit IgG (H&L) (HRP).
The hallmark pathologies of Parkinson’s disease include the loss of dopaminergic neurons from the pars compacta, a portion of the substantia nigra in the midbrain, causing slowness of movement (bradykinesia), resting tremor and rigidity. This is coupled with the presence of intracellular protein aggregates known as Lewy bodies.
The antibodies in this panel were selected for their exceptional performance in imaging applications, alongside other applications. Please see the individual datasheets for additional information.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 packs ab138501 - Anti-alpha Synuclein antibody [MJFR1] 1 x 10µl ab133474 - Anti-LRRK2 antibody [MJFF2 (c41-2)] 1 x 10µl ab76008 - Anti-PARK7/DJ1 antibody [EP2815Y] 1 x 10µl ab216144 - Anti-PINK1 antibody [EPR20730] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H+L (HRP) 1 x 100µg
- Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
- Anti-LRRK2 antibody [MJFF2 (c41-2)] (Alexa Fluor® 647) (ab195023)
- Anti-Alpha-synuclein antibody [MJFR1] (Alexa Fluor® 488) (ab195025)
- Anti-PARK7/DJ1 antibody [EP2815Y] (Alexa Fluor® 488) (ab203989)
- Anti-PARK7/DJ1 antibody [EP2815Y] (Alexa Fluor® 647) (ab204009)
- Anti-Alpha-synuclein antibody [MJFR1] (PE) (ab209306)
- Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (ab209420)
- Anti-PARK7/DJ1 antibody [EP2815Y] (Alexa Fluor® 594) (ab215102)
- Anti-PARK7/DJ1 antibody [EP2815Y] - BSA and Azide free (ab218373)
- Anti-PINK1 antibody [EPR20730] - BSA and Azide free (ab232374)
ab76008, at 1/250 dilution, staining PARK7/DJ1 in human brain by immunohistochemistry using paraffin-embedded tissue.
Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)(+/- treatment with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) cells labeling PINK1 with ab216144 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells treated with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
The negative controls are as follows:
-ve control: PBS, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemical analysis of mouse brain tissue, staining LRRK2 with ab133474.
Antigen retrieval was performed by heat mediation and tissue was blocked with 2% goat serum. Sections were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
IHC image of alpha Synuclein staining in normal Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226767 has not yet been referenced specifically in any publications.