Recombinant Anti-PARM-1 antibody [EPR10009] (ab168369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10009] to PARM-1
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PARM-1 antibody [EPR10009]
See all PARM-1 primary antibodies -
Description
Rabbit monoclonal [EPR10009] to PARM-1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human PARM-1.
-
Positive control
- Human placenta, fetal kidney, fetal heart, and 293T lysates; permeabilized 293 cells; 293 cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10009 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
|
|
IP |
1/100 - 1/500.
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
IP
1/100 - 1/500. |
Target
-
Function
May regulate TLP1 expression and telomerase activity, thus enabling certain prostatic cells to resist apoptosis. -
Tissue specificity
Widely expressed with highest levels in heart, kidney and placenta. -
Sequence similarities
Belongs to the PARM family. -
Post-translational
modificationsHighly N-glycosylated and O-glycosylated. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Endosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 25849 Human
- Entrez Gene: 231440 Mouse
- Entrez Gene: 286894 Rat
- SwissProt: Q6UWI2 Human
- SwissProt: Q923D3 Mouse
- SwissProt: Q6P9X9 Rat
- Unigene: 105460 Human
-
Alternative names
- PARM 1 antibody
- PARM-1 antibody
- PARM1 antibody
see all
Images
-
All lanes : Anti-PARM-1 antibody [EPR10009] (ab168369) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lanes 2-3 : Fetal kidney tissue lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa -
Western blot analysis on pellet from 293 cell lysate immunoprecipitated using ab168369 at a 1/100 dilution, and HRP-conjugated anti-rabbit IgG.
-
Intracellular flow cytometric analysis of permeabilized 293 cells using ab168369 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab168369 has been referenced in 1 publication.
- Lee M et al. Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nat Immunol 15:982-95 (2014). PubMed: 25173345