Key features and details
- Rabbit polyclonal to PARN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARN antibody
See all PARN primary antibodies
DescriptionRabbit polyclonal to PARN
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide, corresponding to a region within amino acids 589-639 of Human PARN (NP_002573.1).
- HeLa, Jurkat and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Function3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Sequence similaritiesBelongs to the CAF1 family.
Contains 1 R3H domain.
Cellular localizationNucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
- Information by UniProt
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
All lanes : Anti-PARN antibody (ab125185) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 3 minutes
ab125185, at 6 µg/mg lysate, detecting PARN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125185 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 3 seconds.
ab125185 has not yet been referenced specifically in any publications.