Key features and details
- Rabbit polyclonal to PARN
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-PARN antibody
See all PARN primary antibodies
DescriptionRabbit polyclonal to PARN
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Cow, Human
Recombinant fragment, corresponding to a region within amino acids 1-256 of Human PARN (Uniprot ID: O95453).
- NIH 3T3 and BCL1 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 73 kDa.|
Function3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Sequence similaritiesBelongs to the CAF1 family.
Contains 1 R3H domain.
Cellular localizationNucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
- Information by UniProt
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
ab154214 has been referenced in 1 publication.
- MacNeil DE et al. N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation. Nucleic Acids Res 47:5368-5380 (2019). PubMed: 30931479