Product nameAnti-PARN antibody
See all PARN primary antibodies
DescriptionRabbit polyclonal to PARN
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Recombinant Human PARN protein (ab114690) can be used as a positive control in WB. This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Sequence similaritiesBelongs to the CAF1 family.
Contains 1 R3H domain.
Cellular localizationNucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
- Information by UniProt
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
All lanes : Anti-PARN antibody (ab27778) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 73 kDa
ab27778, at 1/100 dilution, staining human PARN in HeLa cells.
Image courtesy of Human Protein Atlas
ab27778 staining PARN in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab27778 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab27778 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
This product has been referenced in:
- Shukla S et al. Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects. Nat Struct Mol Biol 23:286-92 (2016). Read more (PubMed: 26950371) »
- Wang X et al. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature 505:117-20 (2014). IF . Read more (PubMed: 24284625) »