Overview

  • Product name

    Anti-PARN antibody [EPR11670(2)]
    See all PARN primary antibodies
  • Description

    Rabbit monoclonal [EPR11670(2)] to PARN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PARN aa 400-500. The exact sequence is proprietary.
    Database link: O95453

  • Positive control

    • K562, Hela, MDA-MB435, PC-12, NIH-3T3 cell lysates, Human papillary adenocarcinoma of thyroid, Mouse liver, Hela cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11670(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/20 - 1/40.
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 73 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the CAF1 family.
    Contains 1 R3H domain.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAN antibody
    • Deadenylating nuclease antibody
    • Deadenylation nuclease antibody
    • PARN antibody
    • PARN_HUMAN antibody
    • Poly A specific ribonuclease antibody
    • Poly(A) specific ribonuclease antibody
    • Poly(A)-specific ribonuclease PARN antibody
    • Polyadenylate specific ribonuclease antibody
    • Polyadenylate-specific ribonuclease antibody
    see all

Images

  • All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MDA-MB-435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa

  • Immunofluorescent analysis of HeLa cells labelling PARN with ab188333 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue). 

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa

  • Immunohistochemical analysis of formalin fixed paraffin embedded Human papillary adenocarcinoma of thyroid labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG.  Counterstained with Haematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of Hela cells fixed in 4% paraformaldehyde labeling PARN with ab188333 at 1/100 and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution.  Counterstained with DAPI (blue).

  • Flow cytometric analysis of Hela cells fixed in 2% paraformaldehyde labeling PARN with ab188333 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution.  Rabbit monoclonal IgG was used as an isotype control.

  • Immunoprecipitation of K562 cell lysate labeling PARN with ab188333 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

References

This product has been referenced in:

  • Son A  et al. PARN and TOE1 Constitute a 3' End Maturation Module for Nuclear Non-coding RNAs. Cell Rep 23:888-898 (2018). ICC/IF ; Human . Read more (PubMed: 29669292) »
  • Moon DH  et al. Poly(A)-specific ribonuclease (PARN) mediates 3'-end maturation of the telomerase RNA component. Nat Genet 47:1482-8 (2015). Read more (PubMed: 26482878) »
See all 2 Publications for this product

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