Product nameAnti-PARP antibody
See all PARP1 primary antibodies
DescriptionRabbit polyclonal to PARP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human PARP1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P09874
- Raji cells. Tonsil.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74290 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 mins, followed by cooling at room temperature for 20 mins.|
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
- Information by UniProt
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
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This product has been referenced in:
- Zhou L et al. Brefeldin A inhibits colorectal cancer growth by triggering Bip/Akt-regulated autophagy. FASEB J 33:5520-5534 (2019). Read more (PubMed: 30668917) »
- Zhang L et al. miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR. Onco Targets Ther 11:5293-5302 (2018). Read more (PubMed: 30214235) »