Anti-PARP antibody, prediluted (ab15496)

Overview

  • Product name
    Anti-PARP antibody, prediluted
    See all PARP1 primary antibodies
  • Description
    Rabbit polyclonal to PARP, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Positive control
    • Raji cells, tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab15496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Working dilution, ready to use for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
    • Sequence similarities
      Contains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • Post-translational
      modifications
      Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
      • ADP ribosyltransferase antibody
      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • ARTD1 antibody
      • msPARP antibody
      • NAD(+) ADP ribosyltransferase 1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • pADPRT 1 antibody
      • pADPRT1 antibody
      • PARP 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly (ADP ribose) polymerase 1 antibody
      • poly (ADP ribose) polymerase family, member 1 antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • poly(ADP ribose) synthetase antibody
      • poly(ADP ribosyl)transferase antibody
      • Poly[ADP ribose] synthetase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      see all

    Images

    • ab15496 staining PARP in human tonsil by Immunohistochemistry (FFPE-sections).

    References

    ab15496 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. It appears that all three of these antibodies recognize the 113kD uncleaved protein, with ab6079 additionally recognizing the 85kD cleaved fragment. Since they all stain for the uncleaved protein, none of the three would...

    Read More

    Thank you for your enquiry. This antibody only reacts with 116 kDa on WB.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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