Product nameAnti-PARP1 antibody [A6.4.12]
See all PARP1 primary antibodies
DescriptionMouse monoclonal [A6.4.12] to PARP1
Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster
Full length native protein (purified) corresponding to Human PARP1.
- Human placenta, skin and spleen tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab110915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/5000.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
- Information by UniProt
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human placenta by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human skin by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human spleen by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
ab110915 has been referenced in 7 publications.
- Xu F et al. Cytoplasmic PARP-1 promotes pancreatic cancer tumorigenesis and resistance. Int J Cancer N/A:N/A (2019). PubMed: 30614530
- Sakthianandeswaren A et al. MACROD2 Haploinsufficiency Impairs Catalytic Activity of PARP1 and Promotes Chromosome Instability and Growth of Intestinal Tumors. Cancer Discov 8:988-1005 (2018). PubMed: 29880585
- Müller AK et al. Proteomic Characterization of Prostate Cancer to Distinguish Nonmetastasizing and Metastasizing Primary Tumors and Lymph Node Metastases. Neoplasia 20:140-151 (2018). PubMed: 29248718
- Nuzzo AM et al. Altered expression of G1/S phase cell cycle regulators in placental mesenchymal stromal cells derived from preeclamptic pregnancies with fetal-placental compromise. Cell Cycle 16:200-212 (2017). WB . PubMed: 27937072
- Viringipurampeer IA et al. NLRP3 inflammasome activation drives bystander cone photoreceptor cell death in a P23H rhodopsin model of retinal degeneration. Hum Mol Genet 25:1501-16 (2016). PubMed: 27008885
- Mohammad G et al. Poly (ADP-ribose) polymerase mediates diabetes-induced retinal neuropathy. Mediators Inflamm 2013:510451 (2013). WB . PubMed: 24347828
- Wysham WZ et al. BRCAness profile of sporadic ovarian cancer predicts disease recurrence. PLoS One 7:e30042 (2012). IHC-P ; Human . PubMed: 22253870