Overview

  • Product name

    Anti-PARP1 antibody [A6.4.12]
    See all PARP1 primary antibodies
  • Description

    Mouse monoclonal [A6.4.12] to PARP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster
  • Immunogen

    Full length native protein (purified) corresponding to Human PARP1.

  • Positive control

    • Human placenta, skin and spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab110915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IP Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT-1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly (ADP-ribose) polymerase 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly(ADP-ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • sPARP 1 antibody
    • sPARP1 antibody
    see all

Images

  • ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human placenta by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.

  • ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human skin by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.

  • ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human spleen by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.

References

This product has been referenced in:

  • Xu F  et al. Cytoplasmic PARP-1 promotes pancreatic cancer tumorigenesis and resistance. Int J Cancer N/A:N/A (2019). Read more (PubMed: 30614530) »
  • Müller AK  et al. Proteomic Characterization of Prostate Cancer to Distinguish Nonmetastasizing and Metastasizing Primary Tumors and Lymph Node Metastases. Neoplasia 20:140-151 (2018). Read more (PubMed: 29248718) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cell line M238)
Permeabilization
Yes - 0.3% Triton
Specification
Melanoma cell line M238
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Methanol

Ms. Hsiao-Wang Chen

Verified customer

Submitted Oct 21 2017

Answer

Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells (Jurkat is recommended for other RIP3 antibodies though). The mouse lung lysate is ab7270 and for the anti-PARP antibody the human spleen is a recommended positive control, we do have this lysate at ab29699.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up