Anti-PARP1 antibody [A6.4.12] (ab110915)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)](https://www.abcam.com/ps/products/110/ab110915/Images/ab110915-124200-anti-parp1-antibody-a6412-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)")
Key features and details
- Mouse monoclonal [A6.4.12] to PARP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PARP1 antibody [A6.4.12]
See all PARP1 primary antibodies -
Description
Mouse monoclonal [A6.4.12] to PARP1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster
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Immunogen
Full length native protein (purified) corresponding to Human PARP1.
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Positive control
- Human placenta, skin and spleen tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
A6.4.12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- SwissProt: P31669 Xenopus laevis
see all -
Alternative names
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)
ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human placenta by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)](https://www.abcam.com/ps/products/110/ab110915/Images/ab110915-124199-anti-parp1-antibody-a6412-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human skin by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)](https://www.abcam.com/ps/products/110/ab110915/Images/ab110915-124198-anti-parp1-antibody-a6412-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [A6.4.12] (ab110915)ab110915, at 10 µg/ml, staining PARP1 in formalin fixed, paraffin embedded Human spleen by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
Datasheets and documents
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Datasheet download
References (9)
ab110915 has been referenced in 9 publications.
- Kashkoulinejad-Kouhi T et al. Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3? co-knockdown. Oncol Rep 45:665-679 (2021). PubMed: 33416155
- Lin HH et al. Overexpression of FAM46A, a Non-canonical Poly(A) Polymerase, Promotes Hemin-Induced Hemoglobinization in K562 Cells. Front Cell Dev Biol 8:414 (2020). PubMed: 32528962
- Xu F et al. Cytoplasmic PARP-1 promotes pancreatic cancer tumorigenesis and resistance. Int J Cancer N/A:N/A (2019). PubMed: 30614530
- Sakthianandeswaren A et al. MACROD2 Haploinsufficiency Impairs Catalytic Activity of PARP1 and Promotes Chromosome Instability and Growth of Intestinal Tumors. Cancer Discov 8:988-1005 (2018). PubMed: 29880585
- Müller AK et al. Proteomic Characterization of Prostate Cancer to Distinguish Nonmetastasizing and Metastasizing Primary Tumors and Lymph Node Metastases. Neoplasia 20:140-151 (2018). PubMed: 29248718
- Nuzzo AM et al. Altered expression of G1/S phase cell cycle regulators in placental mesenchymal stromal cells derived from preeclamptic pregnancies with fetal-placental compromise. Cell Cycle 16:200-212 (2017). WB . PubMed: 27937072
- Viringipurampeer IA et al. NLRP3 inflammasome activation drives bystander cone photoreceptor cell death in a P23H rhodopsin model of retinal degeneration. Hum Mol Genet 25:1501-16 (2016). PubMed: 27008885
- Mohammad G et al. Poly (ADP-ribose) polymerase mediates diabetes-induced retinal neuropathy. Mediators Inflamm 2013:510451 (2013). WB . PubMed: 24347828
- Wysham WZ et al. BRCAness profile of sporadic ovarian cancer predicts disease recurrence. PLoS One 7:e30042 (2012). IHC-P ; Human . PubMed: 22253870
