Anti-PARP1 antibody (ab137653)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Rabbit polyclonal to PARP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PARP1 aa 151-415. The exact sequence is proprietary.
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Positive control
- WB: 293T, HCT116 or HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 113 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 113 kDa. |
Target
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Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
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Alternative names
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
see all
Images
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All lanes : Anti-PARP1 antibody (ab137653) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa
7.5% SDS PAGE -
All lanes : Anti-PARP1 antibody (ab137653) at 1/1000 dilution
Lane 1 : HCT116 whole cell lysate/extract (untreated)
Lane 2 : HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling PARP1 with ab137653 at 1/200 dilution (left), costained with a DNA probe (right).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab137653 has been referenced in 12 publications.
- Tolstik E et al. Raman and fluorescence micro-spectroscopy applied for the monitoring of sunitinib-loaded porous silicon nanocontainers in cardiac cells. Front Pharmacol 13:962763 (2022). PubMed: 36016563
- Sazonova EV et al. Drug toxicity assessment: cell proliferation versus cell death. Cell Death Discov 8:417 (2022). PubMed: 36241623
- Schmidtova S et al. Effect of the PARP inhibitor veliparib on germ cell tumor cell lines. Oncol Lett 24:392 (2022). PubMed: 36276487
- Savelyev NV et al. PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins. Front Cell Dev Biol 9:621134 (2021). PubMed: 34095104
- Streletskaia AY et al. Upregulation of Mcl-1S Causes Cell-Cycle Perturbations and DNA Damage Accumulation. Front Cell Dev Biol 8:543066 (2020). PubMed: 33072738