Overview

  • Product name

  • Description

    Rabbit polyclonal to PARP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PARP1 aa 200-230 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    PGVKSEGKRKGDEVDGVDEVAKKKSKKEKDK


    Database link: P09874

  • Positive control

    • Rat and mouse brain tissues. Rat brain lysates; HeLa and Raji cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab218132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB 1/100 - 1/1000. Predicted molecular weight: 113 kDa.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT-1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly (ADP-ribose) polymerase 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly(ADP-ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • sPARP 1 antibody
    • sPARP1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PARP1 using ab218132 at a 1/200 dilution, followed by conjugation to the secondary antibody goat anti-rabbit IgG, FITC-conjugated at a 1/200 dilution and DAPI.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse brain tissue, labeling PARP1 using ab218132 at a 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • All lanes : Anti-PARP1 antibody (ab218132) at 1/200 dilution

    Lane 1 : Human colon carcinoma lysates
    Lane 2 : rat brain lysates

    Secondary
    All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa
    Observed band size: 112 kDa
    why is the actual band size different from the predicted?

  • Anti-PARP1 antibody (ab218132) at 1/300 dilution + HeLa lysates

    Secondary
    goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa

  • Anti-PARP1 antibody (ab218132) at 1/300 dilution + Human Raji lysates

    Secondary
    goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa

References

This product has been referenced in:

  • Liu Y  et al. Breviscapine ameliorates CCl4-induced liver injury in mice through inhibiting inflammatory apoptotic response and ROS generation. Int J Mol Med 42:755-768 (2018). Read more (PubMed: 29717768) »
See 1 Publication for this product

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