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    parp1-antibody-ab227244.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes ADP-ribosylation
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Anti-PARP1 antibody (ab227244)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (23)

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Western blot - Anti-PARP1 antibody (ab227244)
  • Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody (ab227244)
  • ChIP - Anti-PARP1 antibody (ab227244)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab227244)
  • Immunoprecipitation - Anti-PARP1 antibody (ab227244)
  • Western blot - Anti-PARP1 antibody (ab227244)
  • Western blot - Anti-PARP1 antibody (ab227244)
  • Western blot - Anti-PARP1 antibody (ab227244)
  • ChIP - Anti-PARP1 antibody (ab227244)
  • Western blot - Anti-PARP1 antibody (ab227244)

Key features and details

  • Rabbit polyclonal to PARP1
  • Suitable for: ChIP, IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-PARP1 antibody [EPR18461] (ab191217)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-PARP1 antibody
    See all PARP1 primary antibodies
  • Description

    Rabbit polyclonal to PARP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PARP1 (internal sequence). The exact sequence is proprietary.
    Database link: P09874

  • Positive control

    • WB: HEK-293T, NIH/3T3 and PC-12 whole cell extracts; HCT 116, HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900). ICC/IF: HeLa cells. ChIP: HeLa and Raji chromatin extracts. IP: HCT 116 whole cell extract. IHC-P: HeLa xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human PARP1 protein (ab123834)
  • Related Products

    • Recombinant human PARP1 protein (ab123834)
    • Recombinant human PARP1 protein (ab157046)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP
Use at an assay dependent concentration.
IHC-P
1/100 - 1/1000.
WB (4)
1/500 - 1/10000. Predicted molecular weight: 113 kDa.
ICC/IF (1)
1/100 - 1/1000.
IP (1)
1/100 - 1/500.
Notes
ChIP
Use at an assay dependent concentration.
IHC-P
1/100 - 1/1000.
WB
1/500 - 1/10000. Predicted molecular weight: 113 kDa.
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P09874 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 142 Human
    • Entrez Gene: 11545 Mouse
    • Entrez Gene: 25591 Rat
    • Omim: 173870 Human
    • SwissProt: P09874 Human
    • SwissProt: P11103 Mouse
    • SwissProt: P27008 Rat
    • Unigene: 177766 Human
    • Unigene: 277779 Mouse
    • Unigene: 11327 Rat
    see all
  • Alternative names

    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT-1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly (ADP-ribose) polymerase 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly(ADP-ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • sPARP 1 antibody
    • sPARP1 antibody
    see all

Images

  • Western blot - Anti-PARP1 antibody (ab227244)
    Western blot - Anti-PARP1 antibody (ab227244)
    All lanes : Anti-PARP1 antibody (ab227244) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : PARP1 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 113 kDa



    7.5% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody (ab227244)
    Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody (ab227244)

    4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PARP1 (green) using ab227244 at 1/500 dilution in ICC/IF.

    Red: Phalloidin, a cytoskeleton marker, at 1/200 dilution.

    Blue: Hoechst 33342 staining.

  • ChIP - Anti-PARP1 antibody (ab227244)
    ChIP - Anti-PARP1 antibody (ab227244)

    ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or ab227244. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab227244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab227244)

    Paraffin-embedded HeLa xenograft tissue stained for PARP1 using ab227244 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: EDTA based, pH 8.0, buffer, 15minutes. 

  • Immunoprecipitation - Anti-PARP1 antibody (ab227244)
    Immunoprecipitation - Anti-PARP1 antibody (ab227244)

    PARP1 was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell extract with 4 µg ab227244. Western blot was performed from the immunoprecipitate using ab227244 at 1/500 dilution. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HCT 116 whole cell extract 30 μg.

    Lane 2: Control IP in HCT 116 whole cell extract with 4 μg of preimmune Rabbit IgG.

    Lane 3: ab227244 IP in HCT 116 whole cell extract.

  • Western blot - Anti-PARP1 antibody (ab227244)
    Western blot - Anti-PARP1 antibody (ab227244)
    All lanes : Anti-PARP1 antibody (ab227244) at 1/1000 dilution

    Lane 1 : 293T whole cell extracts
    Lane 2 : A431 whole cell extracts
    Lane 3 : HeLa whole cell extracts
    Lane 4 : HepG2 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution

    Predicted band size: 113 kDa



    5% gel.

    Running conditions: 80V, 15min; 140V, 40 minutes.

    Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).

    Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.

    Primary antibody incubation: 4? , overnight.

    Washing condition: 5 ml TBST, 4 x 5 minutes. 

    Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody

     

  • Western blot - Anti-PARP1 antibody (ab227244)
    Western blot - Anti-PARP1 antibody (ab227244)
    All lanes : Anti-PARP1 antibody (ab227244) at 1/1000 dilution

    All lanes : Neuro2A whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution

    Predicted band size: 113 kDa



    5% gel.

    Running conditions: 80V, 15min; 140V, 40 minutes.

    Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).

    Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.

    Primary antibody incubation: 4? , overnight.

    Washing condition: 5 ml TBST, 4 x 5 minutes. 

    Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody

     

  • Western blot - Anti-PARP1 antibody (ab227244)
    Western blot - Anti-PARP1 antibody (ab227244)
    All lanes : Anti-PARP1 antibody (ab227244) at 1/5000 dilution

    Lane 1 : HCT116 whole cell lysate (untreated)
    Lane 2 : HCT116 whole cell lysate (30 µM cisplatin treatment for 24 hours)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 113 kDa



    7.5% SDS-PAGE gel.

    The top band is full-length and the bottom band on lane 2 is cleaved PARP1. 

  • ChIP - Anti-PARP1 antibody (ab227244)
    ChIP - Anti-PARP1 antibody (ab227244)

    Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or ab227244. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

  • Western blot - Anti-PARP1 antibody (ab227244)
    Western blot - Anti-PARP1 antibody (ab227244)
    All lanes : Anti-PARP1 antibody (ab227244) at 1/500 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 113 kDa



    7.5% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (23)

Publishing research using ab227244? Please let us know so that we can cite the reference in this datasheet.

ab227244 has been referenced in 23 publications.

  • Xue Y  et al. AAV-Txnip prolongs cone survival and vision in mouse models of retinitis pigmentosa. Elife 10:N/A (2021). PubMed: 33847261
  • Xia L  et al. Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells. J Cell Mol Med 25:2404-2417 (2021). PubMed: 33491272
  • Cheng Y  et al. iTRAQ-Based Quantitative Proteomics Indicated Nrf2/OPTN-Mediated Mitophagy Inhibits NLRP3 Inflammasome Activation after Intracerebral Hemorrhage. Oxid Med Cell Longev 2021:6630281 (2021). PubMed: 33628368
  • Meng T  et al. Diabetes Mellitus Promotes Atrial Structural Remodeling and PARP-1/Ikka/NF-?B Pathway Activation in Mice. Diabetes Metab Syndr Obes 14:2189-2199 (2021). PubMed: 34040405
  • Zhang H  et al. Overexpression of HIF-1a protects PC12 cells against OGD/R-evoked injury by reducing miR-134 expression. Cell Cycle 19:990-999 (2020). PubMed: 32266863
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Immunoprecipitation abreview for Anti-PARP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (B-cell lymphocyte whole cell lysate)
Total protein in input
300 µg
Immuno-precipitation step
Protein A
Specification
B-cell lymphocyte whole cell lysate
Read More

Abcam user community

Verified customer

Submitted Mar 13 2019

Western blot abreview for Anti-PARP1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse RPE, retina)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount
30 µg
Specification
mouse RPE, retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Mar 04 2019

Western blot abreview for Anti-PARP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (multiple myeoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
20 nm Bortezomib for 16 hours
Specification
multiple myeoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

Submitted Aug 17 2018

Western blot abreview for Anti-PARP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (U87MG cells)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Treatment
10Gray Irradiation
Specification
U87MG cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

DR. Matthias Dedobbeleer

Verified customer

Submitted Jul 18 2018

Western blot abreview for Anti-PARP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (fibroblast)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 26 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-PARP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 15 2018

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