Key features and details
- Chicken polyclonal to PARP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Product nameAnti-PARP1 antibody
See all PARP1 primary antibodies
DescriptionChicken polyclonal to PARP1
SpecificityIn WB, ab75757 recognizes intact PARP1 (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP1.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human PARP1. Highly purified human PARP1 expressed in baculovirus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 0.316% Tris HCl
Concentration information loading...
Our Abpromise guarantee covers the use of ab75757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.
Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL).
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
- Information by UniProt
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
All lanes : Anti-PARP1 antibody (ab75757) at 1/1000 dilution
Lane 1 : Extract of HeLa cells treated with vehicle
Lane 2 : Extract of HeLa cells treated with staurosporine
Lane 3 : Extract of Jurkat cells treated with vehicle
Lane 4 : Extract of Jurkat cells treated with staurosporine
Lane 5 : Extract of C6 cells treated with vehicle
Lane 6 : Extract of C6 cells treated with camptothecin
Lane 7 : Extract of NIH 3T3 cells treated with vehicle
Lane 8 : Extract of NIH 3T3 cells treated with staurosporine
Lysates/proteins at 20 µg per lane.
All lanes : HRP-conjugated goat anti-chicken IgY at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 1 minute
SDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).
Blocking: in 5% Milk + PBS for 3 hours at RT.
Primary antibody diluted in 5% Milk + PBS and incubated overnight at 4°C.
Secondary antibody diluted in 5% Milk + PBS and incubated for 2 hour at RT.
Predicted band size : 113 kDa: 89 kDa and 24 kDa.
Observed band size : 113 kDa: 89 kDa and 24 kDa.
All lanes : Anti-PARP1 antibody (ab75757) at 1 µg/ml
Lane 1 : Jurkat cell lysate at 5 µg
Lane 2 : Apoptotic Jurkat
cell lysate, induced with camptothecin for 4 hr
All lanes : GAC-HRP at 1/2000 dilution
Predicted band size: 113 kDa
Additional bands at: 89 kDa (possible cleavage fragment)
Note other smaller apoptosis-induced PARP1 fragments in Lane 2.
ab75757 has not yet been referenced specifically in any publications.