Overview

  • Product name
  • Description
    Chicken polyclonal to PARP1
  • Host species
    Chicken
  • Specificity
    In WB, ab75757 recognizes intact PARP1 (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP1.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human PARP1. Highly purified human PARP1 expressed in baculovirus.

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.

Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL). 

ELISA Use at an assay dependent concentration.

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    see all

Images

  • All lanes : Anti-PARP1 antibody (ab75757) at 1/1000 dilution

    Lane 1 : Extract of HeLa cells treated with vehicle
    Lane 2 : Extract of HeLa cells treated with staurosporine
    Lane 3 : Extract of Jurkat cells treated with vehicle
    Lane 4 : Extract of Jurkat cells treated with staurosporine
    Lane 5 : Extract of C6 cells treated with vehicle
    Lane 6 : Extract of C6 cells treated with camptothecin
    Lane 7 : Extract of NIH 3T3 cells treated with vehicle
    Lane 8 : Extract of NIH 3T3 cells treated with staurosporine

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-chicken IgY at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa


    Exposure time: 1 minute


    SDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).

    Blocking: in 5% Milk + PBS for 3 hours at RT.
    Primary antibody diluted in 5% Milk + PBS and incubated overnight at 4°C.
    Secondary antibody diluted in 5% Milk + PBS and incubated for 2 hour at RT.
    Predicted band size : 113 kDa: 89 kDa and 24 kDa.
    Observed band size : 113 kDa: 89 kDa and 24 kDa.

  • All lanes : Anti-PARP1 antibody (ab75757) at 1 µg/ml

    Lane 1 : Jurkat cell lysate at 5 µg
    Lane 2 : Apoptotic Jurkat
    cell lysate, induced with camptothecin for 4 hr

    Secondary
    All lanes : GAC-HRP at 1/2000 dilution

    Predicted band size: 113 kDa
    Additional bands at: 89 kDa (possible cleavage fragment)



    Note other smaller apoptosis-induced PARP1 fragments in Lane 2.

References

ab75757 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react. If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples. We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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