Overview

  • Product name

    Anti-PARP1 antibody [E78]
    See all PARP1 primary antibodies
  • Description

    Rabbit monoclonal [E78] to PARP1
  • Host species

    Rabbit
  • Specificity

    ab32071 should recognise both pro-form and p85 cleaved-form of PARP1.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PARP1 aa 900-1000 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 85, 116 kDa (predicted molecular weight: 113 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
    • Sequence similarities

      Contains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
      • ADP ribosyltransferase antibody
      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • ARTD1 antibody
      • msPARP antibody
      • NAD(+) ADP ribosyltransferase 1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • pADPRT 1 antibody
      • pADPRT-1 antibody
      • pADPRT1 antibody
      • PARP 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly (ADP ribose) polymerase 1 antibody
      • poly (ADP ribose) polymerase family, member 1 antibody
      • Poly (ADP-ribose) polymerase 1 antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • poly(ADP ribose) synthetase antibody
      • poly(ADP ribosyl)transferase antibody
      • Poly(ADP-ribosyl)transferase antibody
      • Poly[ADP ribose] synthetase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      • sPARP 1 antibody
      • sPARP1 antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green ab32071 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32071 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. ab32071 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10 000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PARP1 antibody [E78] (ab32071) at 1/1000 dilution

      Lane 1 : Jurkat cells
      Lane 2 : Jurkat + Staurosporine

      Predicted band size: 113 kDa

    References

    This product has been referenced in:

    • Wang X  et al. LINP1 facilitates DNA damage repair through non-homologous end joining (NHEJ) pathway and subsequently decreases the sensitivity of cervical cancer cells to ionizing radiation. Cell Cycle 17:439-447 (2018). Read more (PubMed: 29527968) »
    • Kim IY  et al. Loperamide overcomes the resistance of colon cancer cells to bortezomib by inducing CHOP-mediated paraptosis-like cell death. Biochem Pharmacol N/A:N/A (2018). Read more (PubMed: 30529689) »
    See all 16 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Oct 10 2016

    Answer

    Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react. If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples. We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    I'm sorry to hear you are experiencing problems with ab32071. You are the only person so far who has had problems with this product. There may be a number of reasons why the antibody is not working at the moment and I would like to offer to look at your protocol in details to see if the problem could be solved with a few modifications. I enclose below a link to a form to help you put your protocol information easily together, https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=32071&mode=questionaire I would also recommend to run a positive control of Jurkat cell lysate to make sure that the problem is not due to low levels of the proteins in HeLa cells. Please be assured that if the problem is due to the quality of the antibody we would be happy to offer you a refund or replacement vial if the antibody was purchased in the last 90 days.

    Read More

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