Immunocytochemistry/ Immunofluorescence abreview for Anti-PARP1 antibody [EPR18461]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (eHAP1)
Permeabilization
Yes - 0.1% TritonX-100
Specification
eHAP1
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti rabbit IgG Alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
We detected signals in nucleus of wild type of eHAP1 cells but not in knockout cells for PARP1. We are happy with the antibody for its specificity and sensitivity.

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Submitted Mar 13 2018

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