Western blot abreview for Anti-PARP1 antibody [EPR18461]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1 cell)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
100000 cells
Specification
HAP1 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/5000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
PARP1 knockout HAP1 cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 110 kDa
Positive control
wild type HAP1 cells

Additional data

Additional Notes
We detected specific bands in wild type HAP1 cell (positive control) but not in PARP1 knock out cells

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Submitted Mar 22 2018

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