Western blot abreview for Anti-PARP1 antibody [EPR18461]

Poor
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
melanoma cell line
Blocking step
(agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% block ace
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: no kDa Non-specific: 120, 40, 35 kDa

Additional data

Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Dr. 令 中野

Verified customer

Submitted Jun 17 2019

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