Key features and details
- Rabbit polyclonal to PARP14
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARP14 antibody
See all PARP14 primary antibodies
DescriptionRabbit polyclonal to PARP14
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PARP14 (N terminal). UniProt ID: Q460N5 isoform 3.
Database link: NM_017554
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Add 200 ul sterile distilled water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab101010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 171 kDa.|
FunctionEnhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity.
Sequence similaritiesContains 3 Macro domains.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain.
Cellular localizationNucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm.
- Information by UniProt
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Anti-PARP14 antibody (ab101010) at 1/1000 dilution + Human fetal brain lysate
Predicted band size: 171 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal colon tissue labelling PARP14 with ab101010 at a dilution of 1/100.
IHC image of PARP14 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101010, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab101010 has been referenced in 1 publication.
- Yan Q et al. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8. Mol Cell Biol 33:845-57 (2013). WB, ICC ; Human . PubMed: 23230272