Overview

  • Product name

    Anti-PARP14 antibody - C-terminal
    See all PARP14 primary antibodies
  • Description

    Rabbit polyclonal to PARP14 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PARP14 (C terminal). The exact sequence is proprietary.
    Database link: Q460N5

  • Positive control

    • WB: U-87 MG, SK-N-SH and SK-N-AS whole cell extracts. IP: SK-N-SH whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 203 kDa.

Target

  • Function

    Enhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity.
  • Sequence similarities

    Contains 3 Macro domains.
    Contains 1 PARP catalytic domain.
    Contains 1 WWE domain.
  • Cellular localization

    Nucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP-ribosyltransferase diphtheria toxin-like 8 antibody
    • ARTD8 antibody
    • B aggressive lymphoma protein 2 antibody
    • BAL2 antibody
    • Collaborator of STAT6 antibody
    • KIAA1268 antibody
    • PAR14_HUMAN antibody
    • PARP 14 antibody
    • PARP-14 antibody
    • PARP14 antibody
    • pART8 antibody
    • Poly (ADP ribose) polymerase family member 14 antibody
    • Poly [ADP-ribose] polymerase 14 antibody
    see all

Images

  • All lanes : Anti-PARP14 antibody - C-terminal (ab229756) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 203 kDa



    5% SDS-PAGE gel.

  • PARP14 was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229756. Western blot was performed from the immunoprecipitate using ab229756. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in SK-N-SH whole cell extract.

    Lane 2: ab229756 IP in SK-N-SH whole cell extract.

References

ab229756 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229756.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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