Key features and details
- Rabbit polyclonal to PARP14 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARP14 antibody - C-terminal
See all PARP14 primary antibodies
DescriptionRabbit polyclonal to PARP14 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human PARP14 (C terminal). The exact sequence is proprietary.
Database link: Q460N5
- WB: U-87 MG, SK-N-SH and SK-N-AS whole cell extracts. IP: SK-N-SH whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 203 kDa.|
FunctionEnhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity.
Sequence similaritiesContains 3 Macro domains.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain.
Cellular localizationNucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm.
- Information by UniProt
- ADP-ribosyltransferase diphtheria toxin-like 8 antibody
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All lanes : Anti-PARP14 antibody - C-terminal (ab229756) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 203 kDa
5% SDS-PAGE gel.
PARP14 was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229756. Western blot was performed from the immunoprecipitate using ab229756. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in SK-N-SH whole cell extract.
Lane 2: ab229756 IP in SK-N-SH whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229756 has not yet been referenced specifically in any publications.