Key features and details
- Goat polyclonal to PARP2
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARP2 antibody
DescriptionGoat polyclonal to PARP2
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
- Human Adrenal andSkin tissues; Human Spleen lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab115620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 66 kDa.|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
Tissue specificityWidely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
Sequence similaritiesContains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
modificationsPoly-ADP-ribosylated by PARP1.
- Information by UniProt
- ADP ribosyltransferase like 2 antibody
- ADP-ribosyltransferase diphtheria toxin-like 2 antibody
- ADPRT 2 antibody
ab115620, at 2.5µg/ml, staining PARP2 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115620, at 2.5µg/ml, staining PARP2 in Formalin-fixed, Paraffin-embedded Human Skin tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-PARP2 antibody (ab115620) at 1 µg/ml + Human Spleen Lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
ab115620 has been referenced in 1 publication.
- Nonomiya Y et al. Novel pharmacological effects of poly (ADP-ribose) polymerase inhibitor rucaparib on the lactate dehydrogenase pathway. Biochem Biophys Res Commun 510:501-507 (2019). PubMed: 30737031