Recombinant Anti-PARP2 antibody [EPR24240-29] (ab271080)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24240-29] to PARP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PARP2 antibody [EPR24240-29]
See all PARP2 primary antibodies -
Description
Rabbit monoclonal [EPR24240-29] to PARP2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HeLa, MDA-MB-231 whole cell lysates; PC-3 whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24240-29 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 66 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 66 kDa. |
Target
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Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. -
Tissue specificity
Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus. -
Sequence similarities
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain. -
Post-translational
modificationsPoly-ADP-ribosylated by PARP1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10038 Human
- Omim: 607725 Human
- SwissProt: Q9UGN5 Human
- Unigene: 409412 Human
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Alternative names
- ADP ribosyltransferase like 2 antibody
- ADP-ribosyltransferase diphtheria toxin-like 2 antibody
- ADPRT 2 antibody
see all
Images
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All lanes : Anti-PARP2 antibody [EPR24240-29] (ab271080) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T(human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : PARP2 knockout HEK-293T whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking and diluting buffer and concentration: 3% NFDM/TBST
Lanes 1-4: Merged signal (red and green). Green - ab271080 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.
ab271080 Anti-PARP2 antibody [EPR24240-29] was shown to specifically react with PARP2 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266414 (knockout cell lysate ab258094) was used. Wild-type and PARP2 knockout samples were subjected to SDS-PAGE. ab271080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-PARP2 antibody [EPR24240-29] (ab271080) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab271080 has not yet been referenced specifically in any publications.