Key features and details
- Rabbit polyclonal to PARP3/IRT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARP3/IRT1 antibody
See all PARP3/IRT1 primary antibodies
DescriptionRabbit polyclonal to PARP3/IRT1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PARP3/IRT1 aa 63-340. Recombinant fragment containing a sequence corresponding to a region within amino acids 63-340 of Human PARP3/IRT1 (NP_005476).
- A431, HeLa S3, 293T, H1299, HepG2, MOLT4 and Raji cell lysates for WB. OVCA tissue for IHC-P.
Protein previously labeled as PARP3.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
Tissue specificityWidely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
Sequence similaritiesContains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
DomainAccording to PubMed:10329013, the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According to PubMed:16924674, it is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected.
- Information by UniProt
- ADP ribosyltransferase (NAD+; poly (ADP ribose) antibody
- ADP ribosyltransferase diphtheria toxin like 3 antibody
- ADPRT-3 antibody
All lanes : Anti-PARP3/IRT1 antibody (ab96601) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
ab96601, at 1/100 dilution staining PARP3/IRT1 in paraffin-embedded OVCA by Immunohistochemistry.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa using ab96601 at 1:500 dilution
ab96601 has been referenced in 1 publication.
- Cao JX et al. MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells. Cell Death Dis 5:e1426 (2014). WB . PubMed: 25255219