Overview

  • Product name

  • Description

    Rabbit polyclonal to PARP3/IRT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PARP3/IRT1 aa 63-340. Recombinant fragment containing a sequence corresponding to a region within amino acids 63-340 of Human PARP3/IRT1 (NP_005476).

  • Positive control

    • A431, HeLa S3, 293T, H1299, HepG2, MOLT4 and Raji cell lysates for WB. OVCA tissue for IHC-P.
  • General notes

    Protein previously labeled as PARP3.

Properties

Applications

Our Abpromise guarantee covers the use of ab96601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
  • Tissue specificity

    Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
  • Sequence similarities

    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
  • Domain

    According to PubMed:10329013, the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.
  • Post-translational
    modifications

    Auto-poly(ADP)-ribosylation.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According to PubMed:16924674, it is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosyltransferase (NAD+; poly (ADP ribose) antibody
    • ADP ribosyltransferase diphtheria toxin like 3 antibody
    • ADPRT-3 antibody
    • ADPRT3 antibody
    • ADPRTL2 antibody
    • ADPRTL3 antibody
    • ARTD3 antibody
    • hPARP 3 antibody
    • hPARP-3 antibody
    • hPARP3 antibody
    • IRT 1 antibody
    • IRT1 antibody
    • NAD(+) ADP ribosyltransferase 3 antibody
    • NAD(+) ADP-ribosyltransferase 3 antibody
    • NAD+ ADP ribosyltransferase 3 antibody
    • pADPRT 3 antibody
    • pADPRT-3 antibody
    • pADPRT3 antibody
    • PARP 3 antibody
    • PARP-3 antibody
    • PARP3 antibody
    • PARP3_HUMAN antibody
    • Poly (ADP ribose) polymerase family, member 3 antibody
    • Poly (ADP ribose) synthetase 3 antibody
    • Poly [ADP-ribose] polymerase 3 antibody
    • Poly[ADP ribose] synthetase 3 antibody
    • Poly[ADP-ribose] synthase 3 antibody
    see all

Images

  • All lanes : Anti-PARP3/IRT1 antibody (ab96601) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa



    7.5% SDS-PAGE
  • ab96601, at 1/100 dilution staining PARP3/IRT1 in paraffin-embedded OVCA by Immunohistochemistry.

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa using ab96601 at 1:500 dilution

References

This product has been referenced in:

  • Cao JX  et al. MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells. Cell Death Dis 5:e1426 (2014). WB . Read more (PubMed: 25255219) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87MG)
Permeabilization
Yes - 0.3% Tri
Specification
U87MG
Blocking step
Goat Serum & BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Dr. Samantha Hopkins

Verified customer

Submitted Aug 30 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Pancreas)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Pancreas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Aug 30 2018

Answer

Thank you for your reply.
A full refund will be no problem. Please could you ask your Finance Department to contact ours, at creditcontrol@abcam.com? My colleagues will be happy to arrange this for you.
Please let me know if you have any further questions.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Loading amount
40 µg
Specification
HeLa cells
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 31 2012

Answer

Thank you for your reply, and I'm happy to issue a credit.
Your credit note ID is *** for original order #***.
I am sorry that this antibody did not work as expected. I have asked our Finance department to issue a credit note for you, which may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
Please let me know if you have any questions or if there is anything else that we can do for you.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Loading amount
40 µg
Specification
Mouse embryonic fibroblasts
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 29 2012

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