Recombinant Anti-PARP4 antibody [EPR8230] (ab133745)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8230] to PARP4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PARP4 antibody [EPR8230]
See all PARP4 primary antibodies -
Description
Rabbit monoclonal [EPR8230] to PARP4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PARP4 aa 1650-1750 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, HepG3, 293T cell lysate; Human fetal kidney tissue lysate; Human fetal liver tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.79 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8230 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa). |
Target
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Tissue specificity
Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas. -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 1 VIT domain.
Contains 1 VWFA domain. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Also found in the nucleus, associated with mitotic spindles. - Information by UniProt
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Database links
- Entrez Gene: 143 Human
- Omim: 607519 Human
- SwissProt: Q9UKK3 Human
- Unigene: 744855 Human
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Alternative names
- 193 kDa vault protein antibody
- 193kDa vault protein antibody
- ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
see all
Images
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All lanes : Anti-PARP4 antibody [EPR8230] (ab133745) at 1/1000 dilution
Lane 1 : HeLa cell lysate at 10 µg
Lane 2 : HepG2 cell lysate at 10 µg
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : 293T cell lysate at 10 µg
Lane 5 : Human fetal liver tissue lysate
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 193 kDa
Observed band size: 193 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133745 has been referenced in 1 publication.
- Prawira A et al. Assessment of PARP4 as a candidate breast cancer susceptibility gene. Breast Cancer Res Treat 177:145-153 (2019). PubMed: 31119570