Product nameAnti-PARP4 antibody [EPR8230]
See all PARP4 primary antibodies
DescriptionRabbit monoclonal [EPR8230] to PARP4
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human PARP4 aa 1650-1750 (C terminal). The exact sequence is proprietary.
- HeLa, HepG3, 293T cell lysate; Human fetal kidney tissue lysate; Human fetal liver tissue lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.79 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).|
RelevanceComponent of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
Cellular localizationCytoplasmic, but is also found in the nucleus, associated with mitotic spindles.
- 193kDa vault protein antibody
- ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
- ADP ribosyltransferase like 1 antibody
All lanes : Anti-PARP4 antibody [EPR8230] (ab133745) at 1/1000 dilution
Lane 1 : HeLa cell lysate at 10 µg
Lane 2 : HepG2 cell lysate at 10 µg
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : 293T cell lysate at 10 µg
Lane 5 : Human fetal liver tissue lysate
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 193 kDa
Observed band size: 193 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133745 has not yet been referenced specifically in any publications.