Key features and details
- Mouse monoclonal [P193-10] to PARP4
- Suitable for: IHC-P, IHC-FoFr
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-PARP4 antibody [P193-10]
See all PARP4 primary antibodies
DescriptionMouse monoclonal [P193-10] to PARP4
Tested applicationsSuitable for: IHC-P, IHC-FoFrmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to amino acids 408-611 of Human PARP4.
- IHC-P: FFPE human kidney tissue sections.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-F: 1/10 - 1/20.
IHC-Fr: 1/10 - 1/20.
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. preferably with ECL
Predicted molecular weight: 193 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityWidely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 1 VIT domain.
Contains 1 VWFA domain.
Cellular localizationCytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Also found in the nucleus, associated with mitotic spindles.
- Information by UniProt
- 193 kDa vault protein antibody
- 193kDa vault protein antibody
- ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
IHC image of PARP4 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24110, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab24110 has been referenced in 2 publications.
- Wu H et al. Single-cell intratumoral stemness analysis reveals the involvement of cell cycle and DNA damage repair in two different types of esophageal cancer. Oncol Rep 41:3201-3208 (2019). PubMed: 31002369
- Li B et al. Conditional Akt activation promotes androgen-independent progression of prostate cancer. Carcinogenesis 28:572-83 (2007). PubMed: 17032658