Overview

  • Product name
    Anti-PARP4 antibody [P193-10]
    See all PARP4 primary antibodies
  • Description
    Mouse monoclonal [P193-10] to PARP4
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 408-611 of Human PARP4.

  • Positive control
    • IHC-P: FFPE human kidney tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab24110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Flow Cyt
IHC-P
IHC-Fr
IHC-FoFr
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IHC-F: 1/10 - 1/20.
    IHC-Fr: 1/10 - 1/20.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. preferably with ECL
    Predicted molecular weight: 193 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Component of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
    • Cellular localization
      Cytoplasmic, but is also found in the nucleus, associated with mitotic spindles.
    • Database links
    • Alternative names
      • 193kDa vault protein antibody
      • ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
      • ADP ribosyltransferase like 1 antibody
      • ADP-ribosyltransferase diphtheria toxin-like 4 antibody
      • ADPRT L1 antibody
      • ADPRTL 1 antibody
      • ADPRTL1 antibody
      • ARTD4 antibody
      • H5 proline rich antibody
      • I alpha I related antibody
      • KIAA0177 antibody
      • Minor vault p193 protein antibody
      • OTTHUMP00000042288 antibody
      • p193 antibody
      • PARP 4 antibody
      • PARP related / IalphaI related H5 / proline rich antibody
      • PARP related antibody
      • PARPL antibody
      • PH 5P antibody
      • PH5P antibody
      • Poly (ADP ribose) polymerase 4 antibody
      • Poly (ADP ribose) polymerase family member 4 antibody
      • Poly (ADP ribose) synthetase antibody
      • Poly (ADP ribosyl) transferase like 1 antibody
      • Poly ADP ribose polymerase 4 antibody
      • Poly ADP ribose polymerase family member 4 antibody
      • Vault 3 antibody
      • Vault antibody
      • Vault poly (ADP ribose) polymerase antibody
      • Vault poly ADP ribose polymerase antibody
      • Vault protein 193 kDa antibody
      • Vault3 antibody
      • von Willebrand factor A domain containing 5C antibody
      • VPARP antibody
      • VWA5C antibody
      see all

    Images

    • IHC image of PARP4 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24110, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Li B  et al. Conditional Akt activation promotes androgen-independent progression of prostate cancer. Carcinogenesis 28:572-83 (2007). Read more (PubMed: 17032658) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been succesful.

    I hope the next experiment is succesful and I would be very pleased to hear how this goes. However, ifyou still have concerns regarding the results, I would like to reassure you that this antibody is tested and covered by our guarantee forWB and human samples. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality records.

    In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



    Order Details
    Antibody code:


    Problem
    Choose: Non-specific band Multiple bands No signal or weak signal High background

    Lot number

    Purchase order number
    or preferably Abcam order number:



    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, wrong band size, more bands, no band etc.)


    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


    Amount of protein loaded


    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Detection method (ECL, ECLPlus etc.)


    Positive and negative controls used (please specify)



    Optimization attempts (problem solving)
    How many times have you tried the Western?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No
    e.g. are the background bands always in the same place?


    What steps have you altered?


    Additional Notes:


    Image:
    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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