Key features and details
- Rabbit polyclonal to PARP7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARP7 antibody
See all PARP7 primary antibodies
DescriptionRabbit polyclonal to PARP7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
- WB: DLD1 cell lysate; Human fetal heart lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa.|
FunctionPoly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
Sequence similaritiesContains 1 C3H1-type zinc finger.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain.
- Information by UniProt
- ADP-ribosyltransferase diphtheria toxin-like 14 antibody
- ARTD14 antibody
- AW558171 antibody
Anti-PARP7 antibody (ab84664) at 1 µg/ml + DLD1 cell lysate at 10 µg
Predicted band size: 76 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Anti-PARP7 antibody (ab84664) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab84664 has been referenced in 1 publication.
- Macpherson L et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin poly(ADP-ribose) polymerase (TiPARP, ARTD14) is a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation. Nucleic Acids Res 41:1604-21 (2013). ICC/IF . PubMed: 23275542