Anti-PARP9 antibody (ab53796)
Key features and details
- Rabbit polyclonal to PARP9
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PARP9 antibody -
Description
Rabbit polyclonal to PARP9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the C terminal region of the short isoform of human PARP9.
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Positive control
- Human Farage non-Hodgkins B-cell lymphoma Human BC-1 B-lymphocyte lymphoma lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody is epitope affinity purified from rabbit antiserum. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 96 kDa).
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IP |
Use at an assay dependent concentration.
Use at a concentration of 7 µg/reaction. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 96 kDa). |
IP
Use at an assay dependent concentration. Use at a concentration of 7 µg/reaction. |
Target
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Function
Involved in inducing the expression of IFN-gamma-responsive genes. -
Tissue specificity
Expressed in lymphocyte-rich tissues, spleen, lymph nodes, peripheral blood lymphocytes and colonic mucosa. Also expressed in nonhematopoietic tissues such as heart and skeletal muscle. Isoform 2 is the predominant form. Most abundantly expressed in lymphomas with a brisk host inflammatory response. In diffuse large B-cell lymphomas tumors, expressed specifically by malignant B-cells. -
Involvement in disease
Note=Overexpressed at significantly higher levels in fatal high-risk diffuse large B-cell lymphomas (DLB-CL) compared to cured low-risk tumors. Overexpression in B-cell lymphoma transfectants may promote malignant B-cell migration. May therefore be involved in promoting B-cell migration and dissemination of high-risk DLB-CL tumors. -
Sequence similarities
Contains 2 Macro domains.
Contains 1 PARP catalytic domain. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Shuttles between the nucleus and the cytosol. Export to the cytosol depends on the interaction with DTX3L. - Information by UniProt
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Database links
- Entrez Gene: 83666 Human
- SwissProt: Q8IXQ6 Human
- Unigene: 518200 Human
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Alternative names
- ADP-ribosyltransferase diphtheria toxin-like 9 antibody
- ARTD9 antibody
- AW214463 antibody
see all
Images
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All lanes : Anti-PARP9 antibody (ab53796) at 1 µg/ml
Lane 1 : Farage (human non-Hodgkin's lymphoma B cell line) cell lysate
Lane 2 : BC-1 (human B-cell lymphoma cell line) cell lysate
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab53796 has been referenced in 4 publications.
- Ma Y et al. Circular RNA PRKCI silencing represses esophageal cancer progression and elevates cell radiosensitivity through regulating the miR-186-5p/PARP9 axis. Life Sci 259:118168 (2020). PubMed: 32739469
- Tao L et al. Long noncoding RNA SNHG16 promotes the tumorigenicity of cervical cancer cells by recruiting transcriptional factor SPI1 to upregulate PARP9. Cell Biol Int 44:773-784 (2020). PubMed: 31774223
- Iwata H et al. PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun 7:12849 (2016). PubMed: 27796300
- Yan Q et al. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8. Mol Cell Biol 33:845-57 (2013). PubMed: 23230272