Key features and details
- Rabbit polyclonal to PARP9
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PARP9 antibody
DescriptionRabbit polyclonal to PARP9
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide derived from the C terminal region of the short isoform of human PARP9.
- Human Farage non-Hodgkins B-cell lymphoma Human BC-1 B-lymphocyte lymphoma lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody is epitope affinity purified from rabbit antiserum.
Our Abpromise guarantee covers the use of ab53796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 96 kDa).|
|IP||Use at an assay dependent dilution. Use at a concentration of 7 µg/reaction.|
FunctionInvolved in inducing the expression of IFN-gamma-responsive genes.
Tissue specificityExpressed in lymphocyte-rich tissues, spleen, lymph nodes, peripheral blood lymphocytes and colonic mucosa. Also expressed in nonhematopoietic tissues such as heart and skeletal muscle. Isoform 2 is the predominant form. Most abundantly expressed in lymphomas with a brisk host inflammatory response. In diffuse large B-cell lymphomas tumors, expressed specifically by malignant B-cells.
Involvement in diseaseNote=Overexpressed at significantly higher levels in fatal high-risk diffuse large B-cell lymphomas (DLB-CL) compared to cured low-risk tumors. Overexpression in B-cell lymphoma transfectants may promote malignant B-cell migration. May therefore be involved in promoting B-cell migration and dissemination of high-risk DLB-CL tumors.
Sequence similaritiesContains 2 Macro domains.
Contains 1 PARP catalytic domain.
Cellular localizationCytoplasm > cytosol. Nucleus. Shuttles between the nucleus and the cytosol. Export to the cytosol depends on the interaction with DTX3L.
- Information by UniProt
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All lanes : Anti-PARP9 antibody (ab53796) at 1 µg/ml
Lane 1 : Farage cell lysate
Lane 2 : BC-1 cell lysate
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
ab53796 has been referenced in 2 publications.
- Iwata H et al. PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun 7:12849 (2016). PubMed: 27796300
- Yan Q et al. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8. Mol Cell Biol 33:845-57 (2013). PubMed: 23230272