Key features and details
- Rabbit polyclonal to Partitioning defective 3-like protein
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Partitioning defective 3-like protein antibody
DescriptionRabbit polyclonal to Partitioning defective 3-like protein
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Partitioning defective 3-like protein aa 1036-1141.
- Human skin tissue
ab122264 is mono-specific.
Previously labelled as PARD3B.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesab122264 is mono-specific.
Our Abpromise guarantee covers the use of ab122264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPutative adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions.
Tissue specificityHighly expressed in kidney, lung and skeletal muscle. Expressed at intermediate levels in brain, heart, placenta, liver and pancreas. Isoform 1 is predominant, while isoform 2 and isoform 3 are expressed at lower levels.
Sequence similaritiesBelongs to the PAR3 family.
Contains 3 PDZ (DHR) domains.
DomainThe N-terminal part (1-351) part blocks the association of the tight junction marker TJP1 with the cell-cell boundary when it is overexpressed.
Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Partially localized along the cell-cell contact region. Colocalizes with TJP1 to epithelial tight junctions.
- Information by UniProt
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Immunocytochemistry/immunofluorescence analysis of human cell line CACO-2 labelling Partitioning defective 3-like protein with ab122264 at 2 µg/mL shows localization to cell junctions.
ab122264, at 1/10 dilution, staining Partitioning defective 3-like protein in paraffin-embedded Human skin tissue by Immunohistochemistry.
ab122264 has been referenced in 1 publication.
- Li T et al. High expression of partitioning defective 3-like protein is associated with malignancy in colorectal cancer. Tumour Biol 39:1010428317698393 (2017). PubMed: 28443499