Anti-Partitioning defective 3-like protein antibody (ab122264)
Key features and details
- Rabbit polyclonal to Partitioning defective 3-like protein
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Partitioning defective 3-like protein antibody -
Description
Rabbit polyclonal to Partitioning defective 3-like protein -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Partitioning defective 3-like protein aa 1036-1141.
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Positive control
- Human skin tissue
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General notes
ab122264 is mono-specific.
Previously labelled as PARD3B.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
ab122264 is mono-specific. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
| IHC-P | 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Putative adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions. -
Tissue specificity
Highly expressed in kidney, lung and skeletal muscle. Expressed at intermediate levels in brain, heart, placenta, liver and pancreas. Isoform 1 is predominant, while isoform 2 and isoform 3 are expressed at lower levels. -
Sequence similarities
Belongs to the PAR3 family.
Contains 3 PDZ (DHR) domains. -
Domain
The N-terminal part (1-351) part blocks the association of the tight junction marker TJP1 with the cell-cell boundary when it is overexpressed. -
Cellular localization
Endomembrane system. Cell junction. Cell junction > tight junction. Partially localized along the cell-cell contact region. Colocalizes with TJP1 to epithelial tight junctions. - Information by UniProt
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Database links
- Entrez Gene: 117583 Human
- SwissProt: Q8TEW8 Human
- Unigene: 657382 Human
- Unigene: 729379 Human
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Alternative names
- ALS2CR19 antibody
- Amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 19 antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 19 protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Partitioning defective 3-like protein antibody (ab122264)
Immunocytochemistry/immunofluorescence analysis of human cell line CACO-2 labelling Partitioning defective 3-like protein with ab122264 at 2 µg/mL shows localization to cell junctions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Partitioning defective 3-like protein antibody (ab122264)ab122264, at 1/10 dilution, staining Partitioning defective 3-like protein in paraffin-embedded Human skin tissue by Immunohistochemistry.
Datasheets and documents
References (1)
ab122264 has been referenced in 1 publication.
- Li T et al. High expression of partitioning defective 3-like protein is associated with malignancy in colorectal cancer. Tumour Biol 39:1010428317698393 (2017). PubMed: 28443499
