Key features and details
- Mouse monoclonal [3C9] to Parvalbumin
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Parvalbumin antibody [3C9]
See all Parvalbumin primary antibodies
DescriptionMouse monoclonal [3C9] to Parvalbumin
Tested applicationsSuitable for: IHC, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Parvalbumin.
Database link: P20472
- WB: Mouse muscle, full length human parvalbumin. IHC: Rat cerebellum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Tissue Culture supernatant.
Our Abpromise guarantee covers the use of ab277625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||1/1000 - 1/5000.
perfusion-fixed with 4% paraformaldehyde for 24 hours, free floating sections
|WB||1/1000 - 1/5000. Predicted molecular weight: 12 kDa.|
FunctionIn muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Sequence similaritiesBelongs to the parvalbumin family.
Contains 2 EF-hand domains.
- Information by UniProt
- D22S749 antibody
- MGC116759 antibody
- Parvalbumin alpha antibody
Perfusion-fixed, free floating sections of rat cerebellum tissue stained for parvalbumin using ab277625 (green) at 1/1000 dilution counterstained with chicken pAb to calbindin (red) at 1/2000 dilution in immunohistochemical analysis. The blue DAPI is staining for nuclear DNA. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear green.
Lanes 2-5 : Anti-Parvalbumin antibody [3C9] (ab277625) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Mouse muscle lysate
Lane 3 : Full length His-tagged recombinant human parvalbumin
Lane 4 : Full length human calretinin
Lane 5 : Full length human calbindin
Predicted band size: 12 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277625 has not yet been referenced specifically in any publications.