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Anti-Parvalbumin antibody (ab11427)

  • Datasheet
  • SDS
Reviews (29)Q&A (4)References (154)

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Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
  • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
  • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
  • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)

Key features and details

  • Rabbit polyclonal to Parvalbumin
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Parvalbumin antibody [EPR13091] (ab181086)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Parvalbumin antibody
    See all Parvalbumin primary antibodies
  • Description

    Rabbit polyclonal to Parvalbumin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Rat Parvalbumin. Purified parvalbumin from rat skeletal muscle.

  • Positive control

    • ICC: U251, HeLa, C6, and rat cordical cells; IHC-P: Human tonsil, cerebellum and skeletal muscle tissue sections.
  • General notes

      

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.1% Sodium azide
    Constituents: 2% BSA, 1.62% Sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Parvalbumin protein (ab101107)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
Human
IHC-P
Human
Application Abreviews Notes
IHC-P (5)
Use a concentration of 1 µg/ml.
ICC/IF (5)
1/100 - 1/200.
Notes
IHC-P
Use a concentration of 1 µg/ml.
ICC/IF
1/100 - 1/200.

Target

  • Function

    In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
  • Sequence similarities

    Belongs to the parvalbumin family.
    Contains 2 EF-hand domains.
  • Target information above from: UniProt accession P20472 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5816 Human
    • Entrez Gene: 25269 Rat
    • Omim: 168890 Human
    • SwissProt: P20472 Human
    • SwissProt: P02625 Rat
    • Unigene: 295449 Human
    • Unigene: 2005 Rat
    • Alternative names

      • D22S749 antibody
      • MGC116759 antibody
      • Parvalbumin alpha antibody
      • PRVA_HUMAN antibody
      • PVALB antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
      Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)

      Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Parvalbumin (green) with ab11427 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
      Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
      Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)

      Immunocytochemistry/Immunofluorescence analysis of C6 (rat glial tumor cell line) cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)
      Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)Image is courtesy of an AbReview submitted by Ms Babben Tinner.

      Immunocytochemcial immunofluorescence analysis of 4% PFA & 0.2% Picric acid fixed rat cordical cells in culture, labelling parvalbumin with ab11427 at a dilution of 1/500 incubated for 12 hours at 4°C in 10mM PBS & 0.03% Triton X diluent blend. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)Image courtesy of an anonymous Abreview.
      ab11427 staining Parvalbumin in human brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11427 at a 1/1000 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/ rabbit IgG.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)

      Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (154)

    Publishing research using ab11427? Please let us know so that we can cite the reference in this datasheet.

    ab11427 has been referenced in 154 publications.

    • Shang Y  et al. Over-expressed MST1 impaired spatial memory via disturbing neural oscillation patterns in mice. Genes Brain Behav N/A:e12678 (2020). PubMed: 32468668
    • Ji MH  et al. Neural network disturbance in the medial prefrontal cortex might contribute to cognitive impairments induced by neuroinflammation. Brain Behav Immun N/A:N/A (2020). PubMed: 32505714
    • Krawchuk MB  et al. Optogenetic assessment of VIP, PV, SOM and NOS inhibitory neuron activity and cerebral blood flow regulation in mouse somato-sensory cortex. J Cereb Blood Flow Metab 40:1427-1440 (2020). PubMed: 31418628
    • Pahle J  et al. Selective Inactivation of Reelin in Inhibitory Interneurons Leads to Subtle Changes in the Dentate Gyrus But Leaves Cortical Layering and Behavior Unaffected. Cereb Cortex 30:1688-1707 (2020). PubMed: 31667489
    • Dionisi C  et al. Primary proprioceptive neurons from human induced pluripotent stem cells: a cell model for afferent ataxias. Sci Rep 10:7752 (2020). PubMed: 32385372
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 33 Abreviews or Q&A

    Immunohistochemistry - Free Floating abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2016

    Immunohistochemistry - Free Floating abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 14 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Common marmoset Tissue sections (Frontal cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Frontal cortex
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Cerebellum
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 25 2019

    Immunohistochemistry free floating abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Coronal brain section)
    Specification
    Coronal brain section
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 21 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse brain froze slice)
    Permeabilization
    Yes - 0.4% Triton-PBS, 15 min
    Specification
    Mouse brain froze slice
    Blocking step
    6% BSA-10% NGS -PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 30 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. J. Y. Seo

    Verified customer

    Submitted Apr 03 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Parvalbumin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (neurons of human cerebral organoid)
    Permeabilization
    Yes - Triton X-100
    Specification
    neurons of human cerebral organoid
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Radhika Menon

    Verified customer

    Submitted Jul 12 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parvalbumin antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Whole brain sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - 0.05% Tween-20
    Specification
    Whole brain sections
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2017

    1-10 of 33 Abreviews or Q&A

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