Anti-Parvalbumin antibody (ab11427)
- Datasheet
- References (100)
- Protocols
Overview
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Product nameAnti-Parvalbumin antibody
See all Parvalbumin primary antibodies -
DescriptionRabbit polyclonal to Parvalbumin
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, WB, ELISA, IHC-P, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Human, Sea urchin
Predicted to work with: Gerbil
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Immunogen
Full length native protein (purified) corresponding to Rat Parvalbumin. Purified parvalbumin from rat skeletal muscle.
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Positive control
- WB: Rat cerebellum extract/ IHC-P: Human tonsil, brain, cerebellum and skeletal muscle tissue. ICC/IF: U-251 MG, HeLa and C6 cells; rat cultured cordical cells; mouse brain cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 3% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab11427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20631843 | |
| IHC-Fr | 1/2000. | |
| WB | Use a concentration of 0.1 µg/ml. Detects a band of approximately 12 kDa. Parvalbumin protein is relatively small and, therefore, it is recommended that electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 µg/ml. | |
| IP | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/100 - 1/200. |
Target
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FunctionIn muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
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Sequence similaritiesBelongs to the parvalbumin family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 5816 Human
- Entrez Gene: 19293 Mouse
- Entrez Gene: 25269 Rat
- Omim: 168890 Human
- SwissProt: P20472 Human
- SwissProt: P32848 Mouse
- SwissProt: P02625 Rat
- Unigene: 295449 Human
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Alternative names
- D22S749 antibody
- MGC116759 antibody
- Parvalbumin alpha antibody
see all
Images
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Immunohistochemistry paraffin embedded sections - Anti-Parvalbumin antibody (ab11427)Mollema, N.J. et al Send to PLoS One. 2011 Mar 8;6(3):e17494. doi: 10.1371/journal.pone.0017494Cell type specific markers do not reveal abnormalities in retinal cell number or localization in shNr1d1 injected mice
Labeling with markers for Blue opsin (blue (S-) opsin expressing cones), Green opsin (green (M-) opsin expressing cones), Rhodopsin (Rod cells), Parvalbumin (amacrine cells), Calbindin (horizontal cells), and Brn3a (ganglion cells) did not reveal any problems with cell placement, organization or morphology within the retina when examined at postnatal day 30.5. n = 3. Scale bar = 50 µm.
Parvalbumin is detected using ab114427 at 1/200 dilution following fixation with methanol:acetic acid (1:3) and embeding with paraffin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Parvalbumin (green) with ab11427 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Western blot - Anti-Parvalbumin antibody (ab11427)Anti-Parvalbumin antibody (ab11427) + rat cerebellum extract -
Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)Immunocytochemistry/Immunofluorescence analysis of C6 cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)Image is courtesy of an AbReview submitted by Ms Babben Tinner.Immunocytochemcial immunofluorescence analysis of 4% PFA & 0.2% Picric acid fixed rat cordical cells in culture, labelling parvalbumin with ab11427 at a dilution of 1/500 incubated for 12 hours at 4°C in 10mM PBS & 0.03% Triton X diluent blend. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.
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Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody (ab11427)This image is courtesy of an anonymous Abreviewab11427 staining mouse brain cells by ICC/IF. Cells were PFA fixed and permeabilized with Triton, prior to blocking with 10% serum for 1 hour at RT. The primary antibody was used undiluted and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)Image courtesy of an anonymous Abreview.ab11427 staining Parvalbumin in human brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11427 at a 1/1000 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/ rabbit IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody (ab11427)Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
References
This product has been referenced in:
- Klug JR et al. Differential inputs to striatal cholinergic and parvalbumin interneurons imply functional distinctions. Elife 7:N/A (2018). Read more (PubMed: 29714166) »
- Willems JGP et al. Parvalbumin interneuron mediated feedforward inhibition controls signal output in the deep layers of the perirhinal-entorhinal cortex. Hippocampus 28:281-296 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29341361) »
