Anti-Parvalbumin antibody (ab32895)
Key features and details
- Goat polyclonal to Parvalbumin
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Parvalbumin antibody
See all Parvalbumin primary antibodies -
Description
Goat polyclonal to Parvalbumin -
Host species
Goat -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Xenopus laevis, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human Parvalbumin aa 99-110 (C terminal).
Sequence:GVDEFSTLVAES
Database link: P20472 -
Positive control
- WB: Human cerebellum and rat brain tissue lysate. Flow Cyt: Kelly cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.01 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Block using milk. Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. |
Flow Cyt |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 0.01 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Block using milk. Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. |
Flow Cyt
Use a concentration of 10 µg/ml. |
Target
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Function
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. -
Sequence similarities
Belongs to the parvalbumin family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 5816 Human
- Entrez Gene: 19293 Mouse
- Entrez Gene: 25269 Rat
- Omim: 168890 Human
- SwissProt: P20472 Human
- SwissProt: P80050 Macaque monkey
- SwissProt: P32848 Mouse
- SwissProt: P02625 Rat
see all -
Alternative names
- D22S749 antibody
- MGC116759 antibody
- Parvalbumin alpha antibody
see all
Images
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Lanes 1-2 : Anti-Parvalbumin antibody (ab32895) at 0.01 µg/ml
Lanes 3-4 : Anti-Parvalbumin antibody (ab32895) at 0.3 µg/ml
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human cerebellum tissue lysate + peptide
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat brain tissue lysate + peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 12 kDaLysate in RIPA buffer. Detected by chemiluminescence.
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Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab32895 has been referenced in 9 publications.
- Cooke JE et al. Contrast gain control occurs independently of both parvalbumin-positive interneuron activity and shunting inhibition in auditory cortex. J Neurophysiol 123:1536-1551 (2020). PubMed: 32186432
- Defourny J et al. Pejvakin-mediated pexophagy protects auditory hair cells against noise-induced damage. Proc Natl Acad Sci U S A 116:8010-8017 (2019). PubMed: 30936319
- Eshima H et al. Dysfunction of muscle contraction with impaired intracellular Ca2+ handling in skeletal muscle and the effect of exercise training in male db/db mice. J Appl Physiol (1985) N/A:N/A (2018). PubMed: 30433865
- Staton CD et al. Orexin 2 receptor stimulation enhances resilience, while orexin 2 inhibition promotes susceptibility, to social stress, anxiety and depression. Neuropharmacology 143:79-94 (2018). PubMed: 30240784
- Damborsky JC et al. Local cholinergic-GABAergic circuitry within the basal forebrain is modulated by galanin. Brain Struct Funct 222:1385-1400 (2017). PubMed: 27496091
- Eshima H et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep 5:N/A (2017). PubMed: 28408640
- Eshima H et al. Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1a. Am J Physiol Regul Integr Comp Physiol 312:R1017-R1028 (2017). PubMed: 28438761
- Iaccarino HF et al. Gamma frequency entrainment attenuates amyloid load and modifies microglia. Nature 540:230-235 (2016). PubMed: 27929004
- Tomer R et al. Advanced CLARITY for rapid and high-resolution imaging of intact tissues. Nat Protoc 9:1682-97 (2014). PubMed: 24945384